Protein Info for IAI46_18485 in Serratia liquefaciens MT49

Annotation: MacB family efflux pump subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 transmembrane" amino acids 274 to 295 (22 residues), see Phobius details amino acids 523 to 548 (26 residues), see Phobius details amino acids 574 to 601 (28 residues), see Phobius details amino acids 615 to 634 (20 residues), see Phobius details PF00005: ABC_tran" amino acids 26 to 174 (149 residues), 110.8 bits, see alignment E=1.3e-35 PF12704: MacB_PCD" amino acids 274 to 493 (220 residues), 150.8 bits, see alignment E=9.7e-48 PF02687: FtsX" amino acids 529 to 643 (115 residues), 78 bits, see alignment E=9.3e-26

Best Hits

Swiss-Prot: 74% identical to MACB_PECAS: Macrolide export ATP-binding/permease protein MacB (macB) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: None (inferred from 91% identity to srr:SerAS9_3646)

Predicted SEED Role

"Macrolide export ATP-binding/permease protein MacB (EC 3.6.3.-)" in subsystem Multidrug Resistance Efflux Pumps (EC 3.6.3.-)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.-

Use Curated BLAST to search for 3.6.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (650 amino acids)

>IAI46_18485 MacB family efflux pump subunit (Serratia liquefaciens MT49)
MASQPLLQLQGISRRFQAGDQNVTVLKEINLSIAQGEMVAIVGASGSGKSTLMNILGCLD
RPSEGDYQVAGRSTARLDRDSLAELRREHFGFIFQRYHLLGDLSAQGNVEVPAVYAGHAR
SARQQRAADLLQRLGLAQRLNYRPSQLSGGQQQRVSIARALMNGGEVILADEPTGALDSH
SGQEVMSILKDLHARGHTVVIVTHDMQVAEHAQRIIELSDGEIVADRRKTTAHTVARLPI
ALDNAVSRWQSQRDRLYEAFKMALLAMASQRLRTLLTMLGIIIGIASVVSVVALGKGSQQ
QVLANINAMGTSTLEVYPGKDFGDMRSAAVQTLRATDADALAQQGYVHSVTPTVSSSTTF
RYGNQSVSGTVNGVGEQYFLVRGYSIDSGMAFNRHSVDQLMQEAVIDENTRNKLFPHGAN
PLGEVIMLGSLPCRIIGVAAKKQSGFGSDENLNVWIPYTTAMKRMLGQSYLKSITVRVND
DIDLTSAEDGVTRLLSQRHGGKDFFVMNTDSIRQTIEKTTATLTLLVSMIALISLVVGGI
GVMNIMLVSVTERTREIGVRMAVGARASDIMQQFLIEAVLVCLLGGTLGVLLSLGIGVVF
SQFSDNFTMVYSTESIVAAFICSTLIGVIFGFFPARRAAGMDPIHALERE