Protein Info for IAI46_18460 in Serratia liquefaciens MT49

Annotation: phosphoenolpyruvate-protein phosphotransferase PtsI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 575 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 2 to 566 (565 residues), 859.5 bits, see alignment E=5.2e-263 PF05524: PEP-utilisers_N" amino acids 3 to 126 (124 residues), 111.2 bits, see alignment E=5.5e-36 PF00391: PEP-utilizers" amino acids 152 to 222 (71 residues), 82.9 bits, see alignment E=1.6e-27 PF02896: PEP-utilizers_C" amino acids 251 to 541 (291 residues), 436.1 bits, see alignment E=9e-135

Best Hits

Swiss-Prot: 87% identical to PT1_ECOLI: Phosphoenolpyruvate-protein phosphotransferase (ptsI) from Escherichia coli (strain K12)

KEGG orthology group: K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] (inferred from 99% identity to srs:SerAS12_3641)

MetaCyc: 87% identical to PTS enzyme I (Escherichia coli K-12 substr. MG1655)
Phosphoenolpyruvate--protein phosphotransferase. [EC: 2.7.3.9]

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.9

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (575 amino acids)

>IAI46_18460 phosphoenolpyruvate-protein phosphotransferase PtsI (Serratia liquefaciens MT49)
MISGILVSPGIAFGKALLLKEDEIVINRKKISADDVEQEVSRFLAGRAKASEQLEAIKTK
AGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDELASADAAAYTIIEGQAKALEELDD
EYLKERAADVRDIGKRLLQNILGLAIVDLSAIQDEVILVATDLTPSETAQLNLDKVLGFI
TDLGGRTSHTSIMARSLELPAIVGTSDVTKQVKNDDYLILDAVNNKIYVNPTADVIDQLK
AEQNQYVTEKNDLAKLKDLPAITLDGHQVEVCANIGTVRDVAGAERNGAEGVGLYRTEFL
FMDRDSLPTEDEQFQAYKAVAEAMGSQAVIVRTMDIGGDKDLPYMNLPKEENPFLGWRAI
RIAMDRREILHAQLRAILRASAFGKLRIMFPMIISVEEVRDLKGEIETLKAQLREESKAF
DETIEVGVMVETPAAAVIAHHLAKEVDFFSIGTNDLTQYTLAVDRGNELISHLYNPMSPS
VLGLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISIPRIKKIIRNT
NFEDVKALAAQALAQPTAQDLMNCVNKFIEEKTLC