Protein Info for IAI46_17735 in Serratia liquefaciens MT49

Annotation: sugar phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00702: Hydrolase
  • amino acids 4 to 175 (172 residues), 92.9 bits, E=7e-30
• TIGR01509: HAD hydrolase, family IA, variant 3
  • amino acids 6 to 181 (176 residues), 63 bits, E=3.8e-21
• PF13419: HAD_2
  • amino acids 7 to 181 (175 residues), 88.2 bits, E=1.5e-28
• PF12710: HAD
  • amino acids 7 to 114 (108 residues), 28.9 bits, E=3e-10
• PF13242: Hydrolase_like
  • amino acids 137 to 182 (46 residues), 31.9 bits, 2.1e-11

Best Hits

Swiss-Prot: 73% identical to HXPA_ECOLI: Hexitol phosphatase A (hxpA) from Escherichia coli (strain K12)

KEGG orthology group: K01112, [EC: 3.1.3.-] (inferred from 94% identity to spe:Spro_3313)

MetaCyc: 73% identical to hexitol phosphatase A (Escherichia coli K-12 substr. MG1655)
Sorbitol-6-phosphatase. [EC: 3.1.3.50]; Sugar-phosphatase. [EC: 3.1.3.50, 3.1.3.23]; Mannitol-1-phosphatase. [EC: 3.1.3.50, 3.1.3.23, 3.1.3.22]; Glycerol-1-phosphatase. [EC: 3.1.3.50, 3.1.3.23, 3.1.3.22, 3.1.3.21]

Predicted SEED Role

"Putative phosphatase YfbT" in subsystem 2-phosphoglycolate salvage

MetaCyc Pathways

• 1,3-propanediol biosynthesis (engineered) (7/9 steps found)
• mannitol cycle (4/5 steps found)
• D-sorbitol biosynthesis I (2/3 steps found)
• mannitol biosynthesis (2/3 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Ascorbate and aldarate metabolism
• Ether lipid metabolism
• Fructose and mannose metabolism
• Glycerolipid metabolism
• Lipopolysaccharide biosynthesis
• Nicotinate and nicotinamide metabolism
• Riboflavin metabolism
• Sphingolipid metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-

Use Curated BLAST to search for 3.1.3.- or 3.1.3.21 or 3.1.3.22 or 3.1.3.23 or 3.1.3.50

Protein Sequence (218 amino acids)

>IAI46_17735 sugar phosphatase (Serratia liquefaciens MT49)
MECKGFLFDLDGTLVDSLPAVERAWVNWAKRRGVDPQDVLDFIHGKQAITSLRHFMPGES
EAAIQQEFLLLEQVEAQDTEGVTALPGAAALLARLNQLDIPWAIVTSGSIPVATARRNAG
GLPQPEVFITAEQVKKGKPQPDAYLLGAERLGLAPQDCVVVEDAAAGILSGLAAGCQVIA
VNAPADAPKLDQVDLLLSSLEQIAVSKTEQGAAINLVA