Protein Info for IAI46_17690 in Serratia liquefaciens MT49

Annotation: NADH-quinone oxidoreductase subunit NuoF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01959: NADH oxidoreductase (quinone), F subunit
  • amino acids 14 to 426 (413 residues), 665.3 bits, E=1.2e-204
• PF01512: Complex1_51K
  • amino acids 55 to 227 (173 residues), 169.4 bits, E=5.4e-54
• PF10589: NADH_4Fe-4S
  • amino acids 342 to 424 (83 residues), 98.5 bits, E=1.7e-32

Best Hits

Swiss-Prot: 90% identical to NUOF_SALTY: NADH-quinone oxidoreductase subunit F (nuoF) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00335, NADH dehydrogenase I subunit F [EC: 1.6.5.3] (inferred from 98% identity to spe:Spro_3304)

MetaCyc: 90% identical to NADH:quinone oxidoreductase subunit F (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain F (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• aerobic respiration III (alternative oxidase pathway) (3/3 steps found)
• NADH to cytochrome bd oxidase electron transfer I (2/2 steps found)
• NAD(P)/NADPH interconversion (4/6 steps found)
• NADH to cytochrome bo oxidase electron transfer I (1/2 steps found)
• aerobic respiration I (cytochrome c) (2/4 steps found)
• Fe(II) oxidation (1/6 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Protein Sequence (450 amino acids)

>IAI46_17690 NADH-quinone oxidoreductase subunit NuoF (Serratia liquefaciens MT49)
MIKDIVRTPEMHPLTWRLRDDKQPVWLDEYRSKNGYAGAEKAIKGLAPDEIVNLVKDAGL
KGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDRLLMEQMPHLLVEGMLIS
AFALKAYRGYIFLRGEYIEAAVHLRRAIAEATEAGYLGKNIMGSGFDFELIVHTGAGRYI
CGEETALINSLEGRRANPRSKPPFPASSGVWGKPTCVNNVETLCNVPAILANGIDWYKGI
SAGKSNDAGTKLMGFSGRVKNPGVWELPFGTTAREILEDYAGGMRDGLRFKAWQPGGAGT
DFLTADHLDLPMDFENIGKAGSRLGTALAMAVDHEIGMVPLVRNLEEFFARESCGWCTPC
RDGLPWSVKILRALENGQGQPGDIETLEQLCRSLGPGKTFCAHAPGAVEPLQSAIKYFRE
EFEAGIAVKHFSNAHAIGGIQPNNLLKQRW