Protein Info for IAI46_17685 in Serratia liquefaciens MT49

Annotation: NADH-quinone oxidoreductase subunit NuoG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 912 PF13510: Fer2_4" amino acids 3 to 77 (75 residues), 41.1 bits, see alignment E=4.5e-14 TIGR01973: NADH dehydrogenase (quinone), G subunit" amino acids 4 to 660 (657 residues), 677.6 bits, see alignment E=1e-207 PF00111: Fer2" amino acids 5 to 71 (67 residues), 30.4 bits, see alignment 9e-11 PF10588: NADH-G_4Fe-4S_3" amino acids 88 to 123 (36 residues), 56.3 bits, see alignment (E = 5.4e-19) PF04879: Molybdop_Fe4S4" amino acids 221 to 274 (54 residues), 45.2 bits, see alignment 2.2e-15 PF00384: Molybdopterin" amino acids 296 to 638 (343 residues), 59 bits, see alignment E=1.3e-19 PF01568: Molydop_binding" amino acids 828 to 894 (67 residues), 27.9 bits, see alignment 6.1e-10

Best Hits

Swiss-Prot: 87% identical to NUOG_YERPE: NADH-quinone oxidoreductase subunit G (nuoG) from Yersinia pestis

KEGG orthology group: K00336, NADH dehydrogenase I subunit G [EC: 1.6.5.3] (inferred from 84% identity to eca:ECA3023)

MetaCyc: 66% identical to NADH-quinone oxidoreductase subunit G (Pseudomonas putida KT2440)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (912 amino acids)

>IAI46_17685 NADH-quinone oxidoreductase subunit NuoG (Serratia liquefaciens MT49)
MATIHVDGKEYDVDGADNLLQACLSLGLDIPYFCWHPALGSVGACRQCAVKQYQNAEDTR
GRLVMSCMTPASDGTFISIDDAEAKQFRESVVEWLMTNHPHDCPVCEEGGNCHLQDMTVM
TGHSFRKYRFSKRTHNNQELGPFISHEMNRCIACYRCVRYYKDYADGTDFGVYGAHDNVY
FGRVESGTLESEFSGNLVEVCPTGVFTDKTHSERYNRKWDMQFAPSICQQCSIGCNTSPG
ERYGELRRIENRYNGSVNHYFLCDRGRFGYGYVNQKDRPRQPQQLRGNDWITLNAEQAMQ
GAADILRQAKKTIGIGSPRASLESNFALRELVGAENFYTGISQAEQGRLNLMLNVLQNSG
VHTPALREIEGYDAVLVLGEDLTQTGARIALSVRQAVKGKARAMAAAQRVADWQIAAIQN
IGQHAKYPLFVTNVDNTRLDDIAAWNYRAPVDEQARLGFAIAHGLDNAAPAVNDLADGLN
KKVDIIVQALTDARKPLIVTGSNAGSEAIIEAAANIAKALKDRGSDVGITFVASAANSIG
LSMIGGGSLDQALSQLENGEADTAIVMENDLYRHAQAAKVDAALAKVSNLIVVDHQRTAI
MDRANLILSAASFAESDGTLVNQEGRAQRFFQVYDPAYYDDAKKDVYTVMLESWRWMHSL
HSTYTSRHVDWTQLDHVIEACVTALPQLQGIVDAAPDASFRIRGQKLARSPHRSSGRTAM
RANISVHEPRQPQDKDTMFAFSMEGNNSPLADRQQIPFAWAPGWNSPQAWNKFQAEVGGK
LRHGDPGVRLIEAGEGKLDYFTQVPAAFDHEQGWRVAPYYHLFGSDEMSQRSGVIQQRMP
EAYVMVNPADAATLGVNAGALVEFSCAGQTLRLPVRLSETLTQGQVGLPLGLPGIPPVLV
GATVENLREATR