Protein Info for IAI46_17255 in Serratia liquefaciens MT49

Annotation: trimethylamine-N-oxide reductase TorA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 826 signal peptide" amino acids 1 to 40 (40 residues), see Phobius details PF18364: Molybdopterin_N" amino acids 61 to 101 (41 residues), 60.5 bits, see alignment 1.8e-20 TIGR00509: molybdopterin guanine dinucleotide-containing S/N-oxide reductases" amino acids 62 to 824 (763 residues), 1147 bits, see alignment E=0 PF00384: Molybdopterin" amino acids 105 to 563 (459 residues), 249.7 bits, see alignment E=6.9e-78 PF01568: Molydop_binding" amino acids 683 to 797 (115 residues), 87.1 bits, see alignment E=1.3e-28

Best Hits

Swiss-Prot: 55% identical to DSTOR_RHOSH: Dimethyl sulfoxide/trimethylamine N-oxide reductase (dmsA) from Rhodobacter sphaeroides

KEGG orthology group: K07812, trimethylamine-N-oxide reductase (cytochrome c) 2 [EC: 1.7.2.3] (inferred from 99% identity to spe:Spro_3233)

MetaCyc: 54% identical to trimethylamine N-oxide reductase 2 (Escherichia coli K-12 substr. MG1655)
Trimethylamine-N-oxide reductase (cytochrome c). [EC: 1.7.2.3]

Predicted SEED Role

"Trimethylamine-N-oxide reductase (EC 1.6.6.9)" in subsystem trimethylamine N-oxide (TMAO) reductase (EC 1.6.6.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.6.9, 1.7.2.3

Use Curated BLAST to search for 1.6.6.9 or 1.7.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (826 amino acids)

>IAI46_17255 trimethylamine-N-oxide reductase TorA (Serratia liquefaciens MT49)
MSKYQQQALKMSRRRFLTGATALAAAPLLAGLWPKSALAQAISQALPQFVVLRQAQKGIL
TGAHWGAFEAIVQDGKMVGVQPVKDDPYPNELITMAPYQVHAENRIKYPMVRKSWLEGGP
GSRTELRGRDEWVRVSWDKAIDLVCGEITRLQKDHGPQSIYAGSYGWKSVGMLHNSRTLL
QRLMNLTGGFLGYAGDYSTGAAQVIMSHVVGSMEVYEQQTAWPNVIENSQLVVLWGCNPM
ITLKNSWNVPDHYGQTGFEALKKKGTRVISIDPVHNDSAKFVDAQWIAPRPYTDGAMLIG
IAHTLLTEKLHNPDFLKTYTAGFDKFQAYLLGETDGTPKTAEWAADVCGVDADVLRQLAR
DMAKNRTMIMGGWGIQRQHHGEQQHWLLVTVAAMLGQIGLPGGGFGFSYHYSSGGSPTAK
GGIISGISAGNAPKNSPAPIPVARIAECLTNPGKTINFNGAQVTYPEVKMVYVAGGNTFH
QHQDTNNLVKAWQRPDTIVVNEPYWTATAKHADIVLPATTSYERNDLEMGGDYSQLYVFP
MHQCVAPQHESRSDFDIFAAMAAKLGVQDAFTEGKDETQWLKGMYDDMKTQARAARVALP
PFDMFWESNNYVRFPVPEANTQWVRFADYRENPLLNPLGTPSGKIEIYSDAIAKMKYQDC
PGIPTWMPPHEWYRGPEAVKYPLSLNTAHPINRLHSQLDNTPLRKKYAVADREAIVIHPQ
DAQSRGIANGDLVRAFNDRGQILVGAVVSEDVRPGVVRISEGAWFDPADPSQPGSLCKNG
NVNCLTFDVGSSSLAQGNCGQMAQLQIEKYQGEVLKNTAHDVPAGA