Protein Info for IAI46_17170 in Serratia liquefaciens MT49

Annotation: FAD-binding oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01494: FAD_binding_3
  • amino acids 27 to 59 (33 residues), 23.6 bits, see alignment (E = 4.2e-09)
• PF01266: DAO
  • amino acids 29 to 380 (352 residues), 246 bits, E=1.3e-76
• PF13450: NAD_binding_8
  • amino acids 32 to 74 (43 residues), 25 bits, 2.9e-09

Best Hits

Swiss-Prot: 74% identical to PUUB_ECOLI: Gamma-glutamylputrescine oxidoreductase (puuB) from Escherichia coli (strain K12)

KEGG orthology group: K09471, gamma-glutamylputrescine oxidase [EC: 1.4.3.-] (inferred from 92% identity to srs:SerAS12_3359)

MetaCyc: 74% identical to gamma-glutamylputrescine oxidase (Escherichia coli K-12 substr. MG1655)
1.4.3.M3 [EC: 1.4.3.M3]

Predicted SEED Role

"Gamma-glutamyl-putrescine oxidase (EC1.4.3.-)"

MetaCyc Pathways

• superpathway of L-arginine and L-ornithine degradation (11/13 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (9/11 steps found)
• superpathway of ornithine degradation (6/8 steps found)
• putrescine degradation II (3/4 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 1.4.3.-

Use Curated BLAST to search for 1.4.3.- or 1.4.3.M3

Protein Sequence (426 amino acids)

>IAI46_17170 FAD-binding oxidoreductase (Serratia liquefaciens MT49)
MTEHVASYYAATANTFAPYPALNEDIRCDVCIIGGGFTGLSSALHLVEAGYDVVVLEAAR
IGWGASGRNGGQVVNSYSRDIDVIEARYGADTAAMLGSMMFEGAEIIRKRIDQYAIACDY
RPGGIFAALNPKQNHALQRQQARWRRYGNNQLEWLDRQAIRREIASDRYVSGLLDRNGGH
LHPLNLALGEADAVRRHGGRLFEQSAATRIDYAEPAKVHTAQGSVSANFVIVAGNAYLGD
RLEPRLSRLSMPCGSQILATEPLSKDMALSLLPNNFCVEDCNYLLDYFRLTADNRLLYGG
GVVYGARDPADIDPLILPKMLKTFPQLKQVRIDYRWSGNFLLTLSRMPQFGRLENNVYYM
QGDSGHGVTCTHLAGKLIAEVLRGDAERFDAFARLPHLPFFGGRQLRVPFTALGAAYYAL
RDRLGF