Protein Info for IAI46_16990 in Serratia liquefaciens MT49

Annotation: citrate lyase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 501 TIGR01584: citrate lyase, alpha subunit" amino acids 22 to 501 (480 residues), 765 bits, see alignment E=1.6e-234 PF04223: CitF" amino acids 36 to 499 (464 residues), 860.4 bits, see alignment E=2e-263

Best Hits

Swiss-Prot: 79% identical to CILA_ECOLI: Citrate lyase alpha chain (citF) from Escherichia coli (strain K12)

KEGG orthology group: K01643, citrate lyase subunit alpha / citrate CoA-transferase [EC: 2.8.3.10 4.1.3.6] (inferred from 97% identity to spe:Spro_3168)

MetaCyc: 79% identical to citrate lyase alpha subunit (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Citrate lyase alpha chain (EC 4.1.3.6)" (EC 4.1.3.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.6

Use Curated BLAST to search for 2.8.3.10 or 4.1.3.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (501 amino acids)

>IAI46_16990 citrate lyase subunit alpha (Serratia liquefaciens MT49)
MKRQQRLMTLNNLADLRCYQDASKANLQARKPRDIKRCDTLEQAVRRSGLQDGMTISFHH
AFRGGDLALNQVMETLAAMGFRNLTLASSSLSECHAPLVGHIRNGVVSRIYTSGLRGPLA
DAISRGLLAEPVQIHSHGGRVNLIESGELAIDVAFLGVPACDEFGNANGYSGEACCGSLG
YAKVDADTAGTVVLLTEAILPYPHHPASLAQDRVDLIVQLERVGDADKIGADATRMTSNP
RELLIARRAAEVIAGSGFFTEGFSLQTGTGGASLAVTRFLEDKMRARGVRAGFALGGITS
TMVDLHEKGLIARLLDVQSFDRAAAMSLARNPNHIEISANQYANFSSKGASVDRLDVVVL
SALEIDTGFNVNVLTGSDGVLRGASGGHCDTAAAARLAIIVAPLVRGRIPTLVEQVTTCV
TPGSSIDILVTDHGIAVNPARPELAQRLQEAGLPVVSIDWLRQRALLLTGEPQPIAFTDK
VVAVVRYRDGSVIDVVHQVAE