Protein Info for IAI46_16170 in Serratia liquefaciens MT49

Annotation: 3-oxoadipate enol-lactonase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02427: 3-oxoadipate enol-lactonase
  • amino acids 3 to 250 (248 residues), 270.4 bits, E=7.1e-85
• PF00561: Abhydrolase_1
  • amino acids 14 to 233 (220 residues), 60.4 bits, E=3.3e-20
• PF12697: Abhydrolase_6
  • amino acids 29 to 239 (211 residues), 42.8 bits, E=1.6e-14
• PF12146: Hydrolase_4
  • amino acids 39 to 223 (185 residues), 43.6 bits, E=3.3e-15

Best Hits

Swiss-Prot: 51% identical to ELH2_ACIAD: 3-oxoadipate enol-lactonase 2 (catD) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

KEGG orthology group: K01055, 3-oxoadipate enol-lactonase [EC: 3.1.1.24] (inferred from 83% identity to spe:Spro_3040)

MetaCyc: 42% identical to subunit of 3-oxoadipate enol-lactone hydrolase (Pseudomonas putida)
3-oxoadipate enol-lactonase. [EC: 3.1.1.24]

Predicted SEED Role

"Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24)" in subsystem Catechol branch of beta-ketoadipate pathway or Chloroaromatic degradation pathway or Protocatechuate branch of beta-ketoadipate pathway (EC 3.1.1.24)

MetaCyc Pathways

• protocatechuate degradation II (ortho-cleavage pathway) (4/4 steps found)
• aromatic compounds degradation via β-ketoadipate (6/9 steps found)
• toluene degradation III (aerobic) (via p-cresol) (7/11 steps found)
• superpathway of salicylate degradation (4/7 steps found)
• catechol degradation III (ortho-cleavage pathway) (3/6 steps found)
• catechol degradation to β-ketoadipate (1/4 steps found)
• 4-methylcatechol degradation (ortho cleavage) (3/7 steps found)
• mandelate degradation to acetyl-CoA (7/18 steps found)
• superpathway of aerobic toluene degradation (14/30 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (15/35 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.24

Protein Sequence (256 amino acids)

>IAI46_16170 3-oxoadipate enol-lactonase (Serratia liquefaciens MT49)
MDIDYRLDGRAAAPLLVLSNSLGTTFDLWQPQLLALSEHFRVLRYNQRGHGATPLSTPRL
RLEDLGQDVIALLDYLDVPSAHFCGISMGGLTGLWLNRYHPQRITRMVVANTAARIGNAE
GWQQRAQQVRSQGLSSIAAASPARWFTEAFLQNHPEQVASLVAALAAGNPEGYAACCDAL
SVADLRADVVSMSRPMRVIAGDADPVTTVADAEFLVGNAPHAELQRLPASHISNLACPQL
FSHSVIEFLTEERSAG