Protein Info for IAI46_16105 in Serratia liquefaciens MT49

Annotation: 4-hydroxy-2-oxovalerate aldolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03217: 4-hydroxy-2-oxovalerate aldolase
  • amino acids 3 to 330 (328 residues), 379.4 bits, E=8e-118
• PF00682: HMGL-like
  • amino acids 5 to 258 (254 residues), 163 bits, E=1.1e-51
• PF07836: DmpG_comm
  • amino acids 268 to 329 (62 residues), 83.7 bits, E=5.3e-28

Best Hits

Swiss-Prot: 87% identical to HOA_SERP5: 4-hydroxy-2-oxovalerate aldolase (mhpE) from Serratia proteamaculans (strain 568)

KEGG orthology group: K01666, 4-hydroxy 2-oxovalerate aldolase [EC: 4.1.3.39] (inferred from 87% identity to spe:Spro_3025)

MetaCyc: 41% identical to 4-hydroxy-2-oxovalerate aldolase (Pseudomonas putida F1)
4-hydroxy-2-oxovalerate aldolase. [EC: 4.1.3.39]

Predicted SEED Role

"4-hydroxy-2-oxovalerate aldolase (EC 4.1.3.39)" (EC 4.1.3.39)

MetaCyc Pathways

• catechol degradation II (meta-cleavage pathway) (6/7 steps found)
• 2-hydroxypenta-2,4-dienoate degradation (3/3 steps found)
• catechol degradation I (meta-cleavage pathway) (4/5 steps found)
• L-tryptophan degradation IX (9/12 steps found)
• L-tryptophan degradation XII (Geobacillus) (9/12 steps found)
• toluene degradation I (aerobic) (via o-cresol) (4/7 steps found)
• toluene degradation V (aerobic) (via toluene-cis-diol) (4/7 steps found)
• meta cleavage pathway of aromatic compounds (6/10 steps found)
• 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation (3/8 steps found)
• p-cumate degradation (3/8 steps found)
• toluene degradation IV (aerobic) (via catechol) (6/13 steps found)
• naphthalene degradation to acetyl-CoA (5/12 steps found)
• p-cymene degradation (4/11 steps found)
• mandelate degradation to acetyl-CoA (7/18 steps found)
• superpathway of aerobic toluene degradation (14/30 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (15/35 steps found)
• superpathway of aromatic compound degradation via 2-hydroxypentadienoate (14/42 steps found)

KEGG Metabolic Maps

• 1,4-Dichlorobenzene degradation
• Benzoate degradation via hydroxylation
• Biphenyl degradation
• Carbazole degradation
• Ethylbenzene degradation
• Fluorene degradation
• Phenylalanine metabolism
• Styrene degradation
• Toluene and xylene degradation

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.39

Use Curated BLAST to search for 4.1.3.39

Protein Sequence (333 amino acids)

>IAI46_16105 4-hydroxy-2-oxovalerate aldolase (Serratia liquefaciens MT49)
MAMLINDSTLRDGQHAVKHQLTVAQLRDYATAVDKTGVAIVEVGHGNGLGASSYQVGRAA
LSDETMLNTVRECLQNSKMGVFMLPGWGTIVDLSKALSCGADVVRIGTHCTEATLAERHL
GWLRDHGAEAHAVLMMSHMATPQQLADQAQLLVEYGAQAVGIMDSAGNLLPQDVTERITA
MRRQVDVPLIFHAHNNLGMAVANSVAAAQAGASVIDGCARGFGAGAGNTQLEVLVPVLER
LGFNTGIDLYHFLDAAELAARELMVTPPMIDSVGIVSGLAGVFSGFKNPVLSHSASAGVD
PRDVFFELGKRQAVAGQEDLIVEVAAQLKRIAL