Protein Info for IAI46_14335 in Serratia liquefaciens MT49
Annotation: bifunctional nicotinamidase/pyrazinamidase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 63% identical to PNCA_ECOLI: Nicotinamidase (pncA) from Escherichia coli (strain K12)
KEGG orthology group: K08281, nicotinamidase/pyrazinamidase [EC: 3.5.1.- 3.5.1.19] (inferred from 88% identity to spe:Spro_2724)MetaCyc: 63% identical to nicotinamidase (Escherichia coli K-12 substr. MG1655)
Amidase. [EC: 3.5.1.4]; Nicotinamidase. [EC: 3.5.1.4, 3.5.1.19]
Predicted SEED Role
"Nicotinamidase (EC 3.5.1.19)" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation or Redox-dependent regulation of nucleus processes (EC 3.5.1.19)
MetaCyc Pathways
- NAD salvage pathway I (PNC VI cycle) (6/7 steps found)
- NAD salvage pathway V (PNC V cycle) (4/5 steps found)
- superpathway of NAD biosynthesis in eukaryotes (10/14 steps found)
- L-arginine degradation X (arginine monooxygenase pathway) (2/3 steps found)
- acrylonitrile degradation I (1/2 steps found)
- indole-3-acetate biosynthesis III (bacteria) (1/2 steps found)
- indole-3-acetate biosynthesis IV (bacteria) (1/2 steps found)
- aldoxime degradation (1/3 steps found)
- superpathway of acrylonitrile degradation (1/3 steps found)
- NAD salvage (plants) (6/11 steps found)
- indole-3-acetate biosynthesis II (5/12 steps found)
KEGG Metabolic Maps
- 1,4-Dichlorobenzene degradation
- Arginine and proline metabolism
- Benzoate degradation via CoA ligation
- Biosynthesis of plant hormones
- Caprolactam degradation
- Cyanoamino acid metabolism
- Glutathione metabolism
- Lipopolysaccharide biosynthesis
- Lysine biosynthesis
- Nicotinate and nicotinamide metabolism
- Nucleotide sugars metabolism
- Phenylalanine metabolism
- Sphingolipid metabolism
- Styrene degradation
- Tryptophan metabolism
- Urea cycle and metabolism of amino groups
Isozymes
Compare fitness of predicted isozymes for: 3.5.1.-, 3.5.1.4
Use Curated BLAST to search for 3.5.1.- or 3.5.1.19 or 3.5.1.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (210 amino acids)
>IAI46_14335 bifunctional nicotinamidase/pyrazinamidase (Serratia liquefaciens MT49) MKTALLLIDLQNDFCPGGALAVTDGDAVIAVANRAIEACNARGEPVVASQDWHPANHRSF AVNANAQVGTLGELEGLPQVWWPVHCVQESPGAELHPQLNQRDIVAVFRKGQNTDIDSYS AFFDNGHRSRTELDRWLKAHDVSHLAVMGLATDYCVKFSVLDALALGYQTSIITDGCRGV NLQPGDSQEALQAMQQAGAKLVTLSQFIER