Protein Info for IAI46_14330 in Serratia liquefaciens MT49

Annotation: asparaginase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00519: L-asparaginase, type I
  • amino acids 3 to 335 (333 residues), 422.7 bits, E=4.7e-131
• PF00710: Asparaginase
  • amino acids 6 to 188 (183 residues), 207.8 bits, E=1.1e-65
• PF17763: Asparaginase_C
  • amino acids 213 to 326 (114 residues), 111.2 bits, E=2.8e-36

Best Hits

Swiss-Prot: 84% identical to ASPG1_ECOLI: L-asparaginase 1 (ansA) from Escherichia coli (strain K12)

KEGG orthology group: K01424, L-asparaginase [EC: 3.5.1.1] (inferred from 98% identity to spe:Spro_2723)

MetaCyc: 84% identical to L-asparaginase 1 (Escherichia coli K-12 substr. MG1655)
Asparaginase. [EC: 3.5.1.1, 3.5.1.38, 3.5.5.4]

Predicted SEED Role

"L-asparaginase I, cytoplasmic (EC 3.5.1.1)" (EC 3.5.1.1)

MetaCyc Pathways

• L-glutamate and L-glutamine biosynthesis (7/7 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• superpathway of L-aspartate and L-asparagine biosynthesis (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-asparagine degradation I (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• L-asparagine degradation III (mammalian) (2/3 steps found)
• N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation (2/5 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Cyanoamino acid metabolism
• D-Glutamine and D-glutamate metabolism
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.1

Use Curated BLAST to search for 3.5.1.1 or 3.5.1.38 or 3.5.5.4

Protein Sequence (338 amino acids)

>IAI46_14330 asparaginase (Serratia liquefaciens MT49)
MQKKSIYVAYTGGTIGMQRSDHGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYAPLIDS
SDMTPEDWQHIADDIKKNYHLYDGFVILHGTDTMAFTASALSFMLENLAKPVIVTGSQIP
LAELRSDGQTNLLNALYLAANHPVNEVSLFFNNKLFRGNRTTKAHADGFDAFASPNLPPL
LEAGIHIRRQNGIDSPVCNGELKVHDITPQPIGVVTIYPGISGAVVRNFLLQPVKALILR
SYGVGNAPQKAELVDELRDASERGIVVVNLTQCISGRVNMEGYATGNALAHAGVISGFDM
TVEAALTKLHYLLSQPLTPEQIRSQMQQDLRGELSISG