Protein Info for IAI46_14310 in Serratia liquefaciens MT49

Annotation: tRNA 2-selenouridine(34) synthase MnmH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 372 TIGR03167: tRNA 2-selenouridine synthase" amino acids 20 to 351 (332 residues), 341.5 bits, see alignment E=2.5e-106

Best Hits

Swiss-Prot: 92% identical to SELU_SERP5: tRNA 2-selenouridine/geranyl-2-thiouridine synthase (selU) from Serratia proteamaculans (strain 568)

KEGG orthology group: K06917, tRNA 2-selenouridine synthase [EC: 2.9.1.-] (inferred from 92% identity to spe:Spro_2719)

MetaCyc: 57% identical to tRNA 2-selenouridine synthase (Escherichia coli K-12 substr. MG1655)
RXN0-2281 [EC: 2.9.1.3]

Predicted SEED Role

"Selenophosphate-dependent tRNA 2-selenouridine synthase" in subsystem Selenocysteine metabolism

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.9.1.- or 2.9.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (372 amino acids)

>IAI46_14310 tRNA 2-selenouridine(34) synthase MnmH (Serratia liquefaciens MT49)
MSTTLKRVDTQDYRRIFLQDIPLIDVRAPVEFQQGAFANAVNLPLMNDDERQAVGTCYKQ
QGQQAAIALGHSLVNGRLREQRTAAWLAQCAQWPEGYLYCFRGGLRSQLVQQWLREAGVT
YSRIIGGYKALRQFLLTTLEQSAELPMVLIGGNTGSGKTVLVNELAEGIDLEGAARHRGS
SFGRTLVAQSGQIDFENRLAVLLLKKQHGGCRRWVLEDEGRIIGSNNLPLPIFNRMQQAP
VAVIDDPFEVRLARLQHEYIDRMRLEFEQALGAEPGWQKYDEYLHHGLFAIRRRLGLERF
QQLTKSLESALQRHYASGNSDAHQEWLVPLLQHYYDPMYHYQLEKKSQRIVFRGNYAEVR
EFLMTYSQNNGE