Protein Info for IAI46_14305 in Serratia liquefaciens MT49

Annotation: DNA topoisomerase III

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 641 TIGR01056: DNA topoisomerase III" amino acids 1 to 636 (636 residues), 934.1 bits, see alignment E=3e-285 PF01751: Toprim" amino acids 3 to 125 (123 residues), 56.5 bits, see alignment E=2.5e-19 PF01131: Topoisom_bac" amino acids 158 to 593 (436 residues), 357.6 bits, see alignment E=1e-110

Best Hits

Swiss-Prot: 83% identical to TOP3_ECOLI: DNA topoisomerase 3 (topB) from Escherichia coli (strain K12)

KEGG orthology group: K03169, DNA topoisomerase III [EC: 5.99.1.2] (inferred from 86% identity to eca:ECA2337)

MetaCyc: 83% identical to DNA topoisomerase III (Escherichia coli K-12 substr. MG1655)
5.99.1.2-RXN [EC: 5.6.2.1]

Predicted SEED Role

"DNA topoisomerase III (EC 5.99.1.2)" in subsystem DNA topoisomerases, Type I, ATP-independent (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.6.2.1 or 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (641 amino acids)

>IAI46_14305 DNA topoisomerase III (Serratia liquefaciens MT49)
MRLFIAEKPSLARAIADVLPKPHRRGDGFIACGNNDVVTWCVGHLLEQAQPDAYDSRYAR
WSLADLPIIPEKWQLQPRPSVSKQLNAIKKLLHEADEVVHAGDPDREGQLLVDEVLDYLA
LTPEKRHSVRRCLINDLNPQAVERAIERLRDNRDFIPLCVSALARSRADWLYGINMTRAY
TILGRNAGYDGVLSVGRVQTPVLGLVVRRDEDIENFVSKDFFEVKAHIVTPKEERFVALW
QPSDSCEPYQDEEGRLLHRPLAEHVLKRIEGQPALVTSYNDKRESDTAPLPFSLSTLQIE
ASKRFNLSAQQVLDVCQRLYETHKLITYPRSDCRYLPEEHFAGRHSVLNAISVHQPDLYP
QPVIDSDRRNRCWDDKKVDAHHAIIPTARASKVSLSQDELNVYGLVARQYLMQFCPDAMF
RKCVIELDIAGGKFIAKARFLAEAGWRTLLGSKERDEENEGAPLPVVAKDDELLCERGEV
VERQTQPPRPFTDASLLSAMTGIARFVQDKALKKILRATDGLGTEATRAGIIELLFKRAF
LYKKGRYIHSSETGRALIHSLPDIAARPDMTANWEATLTQISEKSCRYQDFMQPLVGTLQ
ELIYQAKQSRASMAFRGLPPPPSSGAKKRKKSAGKAREKSE