Protein Info for IAI46_14290 in Serratia liquefaciens MT49

Annotation: exodeoxyribonuclease III

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 268 TIGR00633: exodeoxyribonuclease III (xth)" amino acids 1 to 266 (266 residues), 303 bits, see alignment E=1.9e-94 TIGR00195: exodeoxyribonuclease III" amino acids 1 to 266 (266 residues), 356.5 bits, see alignment E=8.8e-111 PF03372: Exo_endo_phos" amino acids 4 to 259 (256 residues), 127.6 bits, see alignment E=3.1e-41

Best Hits

Swiss-Prot: 81% identical to EX3_ECOLI: Exodeoxyribonuclease III (xthA) from Escherichia coli (strain K12)

KEGG orthology group: K01142, exodeoxyribonuclease III [EC: 3.1.11.2] (inferred from 97% identity to spe:Spro_2715)

MetaCyc: 81% identical to exodeoxyribonuclease III (Escherichia coli K-12 substr. MG1655)
3.1.4.-; 3.1.3.-; Deoxyribonuclease IV (phage-T(4)-induced). [EC: 3.1.21.2]; Exodeoxyribonuclease III. [EC: 3.1.21.2, 3.1.11.2]

Predicted SEED Role

"Exodeoxyribonuclease III (EC 3.1.11.2)" in subsystem DNA repair, bacterial (EC 3.1.11.2)

Isozymes

Compare fitness of predicted isozymes for: 3.1.21.2

Use Curated BLAST to search for 3.1.11.2 or 3.1.21.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (268 amino acids)

>IAI46_14290 exodeoxyribonuclease III (Serratia liquefaciens MT49)
MKFVSFNINGLRARPHQLAAIIEQHQPDVIGLQETKVHDDMFPLEDVSQYGYHVFYHGQK
GHYGVALLTKEKPLAVRRGFPTDEEDAQRRIIMADLATPQGTLTVINGYFPQGESRDHPI
KFPAKTRFYQDLQNYLEQQMSVDSPVLVMGDVNISPSDYDIGIGEENRKRWLRTGKCSFL
PEEREWMDRLLNWGLVDTYRHANPERNDQFSWFDYRSRGFDENRGLRIDLLLASTPLAAR
CIATGIDYDIRGMEKPSDHAPVWAEFSL