Protein Info for IAI46_14035 in Serratia liquefaciens MT49

Annotation: bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00218: IGPS
  • amino acids 6 to 252 (247 residues), 327.5 bits, E=4.7e-102
• PF00697: PRAI
  • amino acids 258 to 448 (191 residues), 202.5 bits, E=5.6e-64

Best Hits

Swiss-Prot: 76% identical to TRPC_SALTY: Tryptophan biosynthesis protein TrpCF (trpC) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K13498, indole-3-glycerol phosphate synthase / phosphoribosylanthranilate isomerase [EC: 4.1.1.48 5.3.1.24] (inferred from 96% identity to spe:Spro_2670)

MetaCyc: 74% identical to fused indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylanthranilate isomerase. [EC: 5.3.1.24]; Indole-3-glycerol-phosphate synthase. [EC: 5.3.1.24, 4.1.1.48]

Predicted SEED Role

"Indole-3-glycerol phosphate synthase (EC 4.1.1.48) / Phosphoribosylanthranilate isomerase (EC 5.3.1.24)" in subsystem Tryptophan synthesis or Auxin biosynthesis (EC 4.1.1.48, EC 5.3.1.24)

MetaCyc Pathways

• superpathway of chorismate metabolism (54/59 steps found)
• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• L-tryptophan biosynthesis (6/6 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.48 or 5.3.1.24

Protein Sequence (453 amino acids)

>IAI46_14035 bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF (Serratia liquefaciens MT49)
MQETVLNKIVRDKAQWVAARQQQQPLASFQNEIVPSERGFYHALQGARTAFILECKKASP
SKGLIRENFDPVEIASVYKDFASAISVLTDEKYFQGSFDFLPLVSKTVTQPVLCKDFIID
PYQIYLARFYQADAILLMLSVLNDDQYRQLAAIAHSLNMGVLTEVISEEELQRAIALEAR
VVGINNRDLRDLSIDLDRTRQLAPRVPHGVTVISESGINNYGQIRELSHFANGFLIGSAL
MSETDLRAAVRRVILGDNKVCGLTRPQDASAAYQAGAVYGGLIFVGRSPRYVDINRAREV
ISGAPLKYVGVFCDAQVETLVKTVERLGLKAVQLHGSEDQAYISALRAELPAKCQIWKAL
SVKDHLPQRDLQHVDRYLLDNGAGGTGQRFDWSVLHGENLENVMLAGGLSADNCVDAAKL
GCAGLDFNSGVESQPGIKDPARLAAVFQTLRAY