Protein Info for IAI46_13305 in Serratia liquefaciens MT49

Annotation: two-component system sensor histidine kinase RstB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 transmembrane" amino acids 6 to 27 (22 residues), see Phobius details amino acids 116 to 133 (18 residues), see Phobius details amino acids 139 to 157 (19 residues), see Phobius details signal peptide" amino acids 28 to 28 (1 residues), see Phobius details PF00672: HAMP" amino acids 159 to 206 (48 residues), 31.8 bits, see alignment 2.2e-11 PF00512: HisKA" amino acids 212 to 270 (59 residues), 34.5 bits, see alignment E=2.5e-12 PF02518: HATPase_c" amino acids 317 to 424 (108 residues), 87 bits, see alignment E=1.8e-28

Best Hits

Swiss-Prot: 70% identical to RSTB_ECOLI: Sensor protein RstB (rstB) from Escherichia coli (strain K12)

KEGG orthology group: K07639, two-component system, OmpR family, sensor histidine kinase RstB [EC: 2.7.13.3] (inferred from 98% identity to srs:SerAS12_2586)

MetaCyc: 70% identical to sensor histidine kinase RstB (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"Sensory histidine kinase in two-component regulatory system with RstA" in subsystem Orphan regulatory proteins

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (434 amino acids)

>IAI46_13305 two-component system sensor histidine kinase RstB (Serratia liquefaciens MT49)
MRKLFVQFFLLLFVCFLVMAMLVGLVYKVTAERAGRQSMDDLMKSSLYLMRSELREIPLK
DWNKTIATLDLNLSFKLHIEPLGKQDLSEDLKKRLRLGEIIALDDQYTFMQRIPRSHYVL
VVGPIPYLFYLHQMRLLDLALLVFIGMSLALPVFLWMRPHWQDLLKLENAAQRLGAGHLD
ERTHFDPTSSLNRLGVAFNQMADNVNTLIASKKQLIDGIAHELRTPLVRLRYRLAMSDNL
SDSEQQALNRDIGQLESLIDELLTYARLDRPQVALNIEPLDLPKWLEDKADDLRLIHPER
EIQLDIPHVGDFGGVDLRLMERVLDNLVNNALRYSEQRLRIGLWFDGDSACLQVEDDGPG
IPPEERERVFEPFVRLDPSRDRATGGCGLGLAIVHSIAVAYQGQVYVDASSLGGASFRFC
WPIKPTFDLKADPV