Protein Info for IAI46_13115 in Serratia liquefaciens MT49

Annotation: Zn-dependent hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01879: amidase, hydantoinase/carbamoylase family
  • amino acids 8 to 403 (396 residues), 429.7 bits, E=5.4e-133
• PF01546: Peptidase_M20
  • amino acids 79 to 403 (325 residues), 99.9 bits, E=1.9e-32
• PF07687: M20_dimer
  • amino acids 208 to 309 (102 residues), 23.5 bits, E=4.6e-09

Best Hits

KEGG orthology group: K06016, N-carbamoyl-L-amino-acid hydrolase [EC: 3.5.1.87] (inferred from 90% identity to spe:Spro_2555)

Predicted SEED Role

"Beta-ureidopropionase (EC 3.5.1.6)" in subsystem Hydantoin metabolism or L-2-amino-thiazoline-4-carboxylic acid-Lcysteine conversion or Pyrimidine utilization (EC 3.5.1.6)

MetaCyc Pathways

• superpathway of pyrimidine ribonucleosides degradation (3/5 steps found)
• thymine degradation (1/3 steps found)
• uracil degradation I (reductive) (1/3 steps found)

KEGG Metabolic Maps

• Drug metabolism - other enzymes
• Pantothenate and CoA biosynthesis
• Pyrimidine metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.87

Use Curated BLAST to search for 3.5.1.6 or 3.5.1.87

Protein Sequence (408 amino acids)

>IAI46_13115 Zn-dependent hydrolase (Serratia liquefaciens MT49)
MTHRVNGERLWQSLLDMAQFGAIPKDGVTRLALSEEDRMARNQLREWALEAGCSVRIDRM
GNMFLRREGTRPDLAPVVTGSHVDSQPNGGRFDGIYGVLAGLEVIRTLNDRQIATERAVE
VINWTNEEGARFAPAMISSGVFAGVFELEYGLSRQDAQGTSIGEALQQIGYAGEHPVGNT
PIHAAFELHIEQGPILEAENIEIGVVTAAQGQRWYELEITGFSAHAGTTPMDRRRDALLG
FAAMVKAVNDIGNDFMPDARATVGMAQITPNSRNVVPGKVFFSVEFRHPQEAVLAQMEQR
LLAAVAEVGVDGLNAHAERIFQYQPIRFDPACIDSVRQAAKTLGYSHRDMVSGAGHDACY
LNRVAPTAMIFIPCVDGISHNELENISPAWSTAGANVLLNAILAQTHA