Protein Info for IAI46_12925 in Serratia liquefaciens MT49

Annotation: alpha-glucosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 564 PF00128: Alpha-amylase" amino acids 32 to 381 (350 residues), 402.3 bits, see alignment E=3.5e-124 PF11941: DUF3459" amino acids 467 to 553 (87 residues), 22.1 bits, see alignment E=2.5e-08 PF16657: Malt_amylase_C" amino acids 484 to 554 (71 residues), 29.2 bits, see alignment E=1.2e-10

Best Hits

Swiss-Prot: 56% identical to O16G3_BACSU: Probable oligo-1,6-glucosidase 3 (yugT) from Bacillus subtilis (strain 168)

KEGG orthology group: K01187, alpha-glucosidase [EC: 3.2.1.20] (inferred from 89% identity to spe:Spro_2521)

Predicted SEED Role

"Alpha-glucosidase (EC 3.2.1.20)" in subsystem D-Galacturonate and D-Glucuronate Utilization or Maltose and Maltodextrin Utilization (EC 3.2.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.20

Use Curated BLAST to search for 3.2.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (564 amino acids)

>IAI46_12925 alpha-glucosidase (Serratia liquefaciens MT49)
MSGGQTVQWWKQAVVYQIYPRSFMDSNGDGIGDLNGITAKLDYLQWLGIDVIWICPMYRS
PNDDNGYDISDYQAIMTEFGTMDDFDRLLAEVHARGMRLILDLVVNHTSDEHPWFIESRS
SQASAKRDWYIWRDGKNGAEPNNWESIFNGSAWKYDPGSEQYFLHLFSERQPDLNWENPQ
VRAALYDMMRWWLDKGIDGFRIDAICHMKKQPGLTDMPNPLGLRYVPSFERHLNYEGLLE
YVDDLCEQVFNRYDIMTVGEMNGASAEQGEDWVGEQHGRLNMIFQFEHVKLWENNHQQSP
QAGADVLGLKDIFTRWQTLLEGKGWNALYVENHDIPRVVSKWGDDKQYWRESATAIAAMY
FLMQGTPFIYQGQELGMTNTRFDSLADFNDIAARNRFAKLREQGMDEAQILAFLGRSGRD
NSRTPMQWDDSANAGFSSATPWFAVNANYPQINVARQREEADSVLNFYRALIRLRKQDPV
WIEGRYQLQLAEHPHIYAYSRTVGTQQRLVLCNLSGEPQQVDPQAMPAGPWQWLLGNYQD
NGEWLTLRPYEARIYQQAEATEGI