Protein Info for IAI46_12730 in Serratia liquefaciens MT49
Annotation: type II asparaginase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 75% identical to ASPG_DICCH: L-asparaginase (ansB) from Dickeya chrysanthemi
KEGG orthology group: K01424, L-asparaginase [EC: 3.5.1.1] (inferred from 93% identity to srs:SerAS12_2462)MetaCyc: 82% identical to N-(1-deoxy-D-fructos-1-yl)-L-asparaginase (Salmonella enterica enterica serovar Typhimurium str. LT2)
Asparaginase. [EC: 3.5.1.1]
Predicted SEED Role
"L-asparaginase (EC 3.5.1.1)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 3.5.1.1)
MetaCyc Pathways
- superpathway of L-aspartate and L-asparagine biosynthesis (4/4 steps found)
- L-asparagine degradation I (1/1 steps found)
- L-asparagine degradation III (mammalian) (2/3 steps found)
- N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation (2/5 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.5.1.1
Use Curated BLAST to search for 3.5.1.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (349 amino acids)
>IAI46_12730 type II asparaginase (Serratia liquefaciens MT49) MFQTIRVALICFTFSFAGVASAAGDLPHIVILATGGTIAGSAASNTQTTGYKAGALGVET LIAAVPEMQKVARVDGEQIANIGSENMTSDILLKLSKRVNELLSRSDVDGVVITHGTDTL DESPYFLNLTVKSNKPVVFTAAMRPATAISADGPMNLLDAVTVAADKQSVGRGVMVVLND RIGSARFSTKTNATTLDTFKAPEEGYLGVIVGGKPYFETRVDKIHTTRSVFDVRDVKVLP KVDIIYGYQDDPEYMYDAAIQHKVDGIVYAGTGAGSVSVRSSAGIKKAEKAGVLVVRASR TGSGIVPEDSGQPGLVSDSLNPAKARILLMLSLTKTHNPRVIQDYFHTY