Protein Info for IAI46_12710 in Serratia liquefaciens MT49

Annotation: efflux RND transporter permease subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1045 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details amino acids 340 to 359 (20 residues), see Phobius details amino acids 366 to 388 (23 residues), see Phobius details amino acids 395 to 416 (22 residues), see Phobius details amino acids 438 to 459 (22 residues), see Phobius details amino acids 471 to 497 (27 residues), see Phobius details amino acids 538 to 556 (19 residues), see Phobius details amino acids 873 to 891 (19 residues), see Phobius details amino acids 898 to 918 (21 residues), see Phobius details amino acids 924 to 945 (22 residues), see Phobius details amino acids 973 to 993 (21 residues), see Phobius details amino acids 1000 to 1026 (27 residues), see Phobius details TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1041 (1041 residues), 1569.6 bits, see alignment E=0 PF00873: ACR_tran" amino acids 1 to 1028 (1028 residues), 1247.4 bits, see alignment E=0 PF03176: MMPL" amino acids 331 to 496 (166 residues), 28.1 bits, see alignment E=1.5e-10 PF02355: SecD_SecF" amino acids 340 to 496 (157 residues), 23.1 bits, see alignment E=6.5e-09

Best Hits

Swiss-Prot: 58% identical to ACRB_ECOLI: Multidrug efflux pump subunit AcrB (acrB) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 61% identity to ant:Arnit_3082)

MetaCyc: 58% identical to multidrug efflux pump RND permease AcrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN0-592

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1045 amino acids)

>IAI46_12710 efflux RND transporter permease subunit (Serratia liquefaciens MT49)
MAQFFIRRPVFAWVIAIIIMLCGLMSINSLPISQYPDVSPPQISISATYPGADAETLENS
VTQVIEQQLTGLDGLLYFSATSSSDGSVSINVTFDQGTDPDIAQVQVQNKVQQAESRLPT
EVTAQGVTVKKSQSSFLLIVGLYDETDKASMADISDYLVSNIQDPLSRIEGVGDVQVFGS
EYAMRIWLDPTKLASFSLMPSDVSTAIEAQNTQVSAGKVGDLPSGADQQLTATVKAQSRL
QTPEQFRNIILKSDSSGALVRLGDVARVELGNEDYSSSARLNGHPASGIAVKLAAGANAL
NTAKLVKAKVAEYQTSMPNGYKVAYPKDSTDFINISIEEVVKTLIEAVVLVVIVMFVFLQ
NLRTTLIPTITVPVVLLGTFGVLAAFGYSINTLTLFGMVLAIGLLVDDAIVVVENVERVM
REEKLPPREATEKSMKEITGALVGIAMVLSAVFLPMAFFGGSTGVIYRQFSVTIVASMVL
SVILALTLAPALCATLLKPAHDDLTDKGLLGKFNRGYNRLQEKYADKVARVIHSPMRYLL
LYGLLLIAAAVMYMRLPTGFLPNEDQGVVMVQYTMPAGATDARTSAVSDAVKDYFLTDEK
ANVSTIFTINGFGFSGSGQNAGMAFVSLKDWSQRPGSANTADAIAKRAMAHFATIRDAQV
FSMSPPAIDGFGQSDGFTFELQAKGATTRAELQVMRDQIIAAAGKNPSLSAVRANTLPDT
PQLQVDIDNDKLQALGLSAGDVSSTLTSAFGSTYINDFIDRNRVKKVYMQGDVEYRSKPE
DLDKWFVRGTSSSTTSMTPFSAFASSHWIYGPENLSRYNGLSSFEITGEGAPGTSSGTAM
DEMEKLASQFSSASSFSWSGLSYQERLTSGQAASLYAISLVVVFLCLAALYESWSIPFAV
MMVVPMGVVGSLLAITLRGLENDIYFQVALLTIIGLSAKNAILIVEFAEENYQRGEALIA
AAVEASRMRLRPILMTSLAFSAGVLPLAVSSGAGANSRIAIGTGIIGGTITATLLAIFFV
PLFYVLVRRMFSRKTTENTAPQAGE