Protein Info for IAI46_12490 in Serratia liquefaciens MT49

Annotation: formate dehydrogenase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04879: Molybdop_Fe4S4
  • amino acids 1 to 53 (53 residues), 53.5 bits, 2.9e-18
• TIGR01591: formate dehydrogenase, alpha subunit
  • amino acids 6 to 685 (680 residues), 911.6 bits, E=1.2e-278
• PF00384: Molybdopterin
  • amino acids 61 to 488 (428 residues), 270.7 bits, E=2.8e-84
• PF01568: Molydop_binding
  • amino acids 576 to 683 (108 residues), 99.2 bits, E=2.1e-32

Best Hits

Swiss-Prot: 66% identical to FDHF_ECOLI: Formate dehydrogenase H (fdhF) from Escherichia coli (strain K12)

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 97% identity to spe:Spro_2424)

MetaCyc: 66% identical to formate dehydrogenase H (Escherichia coli K-12 substr. MG1655)
RXN0-3281 [EC: 1.17.98.4]; FHLMULTI-RXN [EC: 1.17.98.4]

Predicted SEED Role

"Formate dehydrogenase H (EC 1.2.1.2)" in subsystem Formate dehydrogenase (EC 1.2.1.2)

MetaCyc Pathways

• mixed acid fermentation (16/16 steps found)
• formate oxidation to CO₂ (1/1 steps found)
• hydrogen production V (1/1 steps found)
• purine nucleobases degradation II (anaerobic) (17/24 steps found)
• oxalate degradation VI (1/4 steps found)
• methanol oxidation to carbon dioxide (2/6 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)
• oxalate degradation III (1/5 steps found)
• superpathway of C1 compounds oxidation to CO₂ (4/12 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Methane metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.17.98.4 or 1.2.1.2

Protein Sequence (718 amino acids)

>IAI46_12490 formate dehydrogenase subunit alpha (Serratia liquefaciens MT49)
MKKITTVCPYCGAGCKMKLVVDNGKIIRAEAADGVTNQGELCLKGYYGWDFLNDTKLLTP
RLSQPLIRRQKGGKFEAVSWDEAISYTAQRLQQIKEQHGASAIMHTGSSRGTGNETNYVM
QKFARAVIGTNNVDCCARVCHGPSVAGLQATLGNGAMSNSIGDIENSKCLLIIGYNCADS
HPIVARRVLKAKEKGAQIVVCDPRRIETARIADQHLQIKNGCNMALVNAFAYVLIEENLY
DRDYVAKYTEGFEAYRQQLADYAPEAVEHLTGVTAQQIRQAMRTYASAPSATIMWGMGVT
QFGQAVDVVKGLASLALLTGNLGRANVGVGPVRGQNNVQGACDMGVLPNEFPGYQAVTDA
AVRAKFAAAWGIDATKMDPNVGYRITEIPHLVHEGKVKAYYIMGEDPLQTEADLSLVRSA
FEALEFVVVQDIFMTKTAEQADVILPATSWGEHGGVFSCADRGFQRFEKAIEPQYNVKRD
WEIISLLASEMGYPMHYEDNQQIWDEMRELCPLFYGATYEKMGELGHVQWPCTSLESQGT
PYLYQGNQFTTPSGKGQLFATTWRAPAEIPDADYPLVLCTVREVGHYSCRSMTGNCAALQ
TLADEPGFVQINPQDAAALGIADQQLVWVASRRGKVISRANYNERINLGAVYMTYQWWIG
ACNELTQENLDPISKTPETKYCAVKLAAITDQHWAENYAQQSYSDMKARLRRAAEDVM