Protein Info for IAI46_12320 in Serratia liquefaciens MT49

Annotation: sigma-54-dependent Fis family transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 596 PF00158: Sigma54_activat" amino acids 312 to 467 (156 residues), 198 bits, see alignment E=2.3e-62 PF14532: Sigma54_activ_2" amino acids 322 to 472 (151 residues), 51.4 bits, see alignment E=3.5e-17 PF07728: AAA_5" amino acids 324 to 442 (119 residues), 23.5 bits, see alignment E=1.3e-08 PF02954: HTH_8" amino acids 561 to 591 (31 residues), 40.9 bits, see alignment (E = 3.5e-14)

Best Hits

KEGG orthology group: None (inferred from 85% identity to spe:Spro_2411)

Predicted SEED Role

"Formate hydrogenlyase transcriptional activator" in subsystem Formate hydrogenase

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (596 amino acids)

>IAI46_12320 sigma-54-dependent Fis family transcriptional regulator (Serratia liquefaciens MT49)
MLHKDQTTQTGCISDDDRLSLSGPLSESWLRSRHYGLNRSDDHVPFIQPTLLKEVRGQNG
WVAQLARPLIERLGGEINRQPSIVVVSDAGGLVLETCGNTHFLRKASRVSLAPGNLWGEQ
ERGTNAIGTALAMGALCEVNGDEHFLNQNAGLYCSAAPIYRPDGLIAGVLDISTPAQHPY
SEAGSLILQAVRHIEHQWVKSCVTDRHWTLRLHSDARVLGSAHEMILVFRDEILTAANRL
AMQEFNLSAAAFGSMDFATLFPEMSLQTMNAPRQTLAVNNRHYYSLLQMPERPSQCVRQQ
LQPYDRDGADRQKALRILNAGLALCISGETGCGKEYFSQRLFEESHRRQGNFVAINCAAL
PESLIESELFGYAPGAFTGASTKGYVGKIREADGGVLFLDEIGDMPLSLQTRLLRVLQEK
TVTPLGSRVSYAVDFSLICATHQDLEQRVAAGAFREDLLYRIQEFSLRILPLRQRAHVDR
FILNLWSELGGDARGIRLAPDAITALAGYSWPGNVRQLLSTLKVLLALADDGNLIRLEDL
PEQFHLLARPVAPQSVSVDMLTAIRNAKGNISLAAKRLGISRSTLYRKMEKHRAES