Protein Info for IAI46_12095 in Serratia liquefaciens MT49

Annotation: LLM class flavin-dependent oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 445 TIGR03860: FMN-dependent oxidoreductase, nitrilotriacetate monooxygenase family" amino acids 14 to 429 (416 residues), 553.4 bits, see alignment E=1.6e-170 PF00296: Bac_luciferase" amino acids 28 to 387 (360 residues), 158.8 bits, see alignment E=1.1e-50

Best Hits

Swiss-Prot: 47% identical to YXEK_BACSU: Putative monooxygenase YxeK (yxeK) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 97% identity to srr:SerAS9_2324)

MetaCyc: 47% identical to N-acetyl-S-(2-succino)-L-cysteine monooxygenase monomer (Bacillus subtilis subtilis 168)
1.14.13.M85 [EC: 1.14.13.M85]

Predicted SEED Role

"Nitrilotriacetate monooxygenase component A (EC 1.14.13.-)" (EC 1.14.13.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.13.-

Use Curated BLAST to search for 1.14.13.- or 1.14.13.M85

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (445 amino acids)

>IAI46_12095 LLM class flavin-dependent oxidoreductase (Serratia liquefaciens MT49)
MSTSPSTPSRQLRLGLFVQALGHHVGGWRAEGASGSPTDIDWFTWIAQKAEEGTFDMFFV
GDALATSVHRLPSTLSRLEPLTLLAALAVRTRHIGLAATASTTFDQPFHLARAMASIDHI
SHGRAAWNVVTSFSSDAARNFSRDDLPSHAERYEVAREFLEASYKLWDGWEEGAIVRDKQ
SGVYAIDEKIHAANHQGKHFSVQGPLNIARSPQGRPVIIEAGSSPAGQQLAAETAEVVFT
AAATLEEGQAFYRSQKKFVADAGRNPDHLLILPGVMPIVGRTKAEAQETWYQLNQLVDID
NGIEQLSARFGFDLSGFPLDGPVPDVGATEGGQSRVKLLTDLAARENLTLRELAAVAAGS
RGHRVVVGTAEEIADDFQLWLEQQGADGFNIMPAVLPNQLELFVELVIPELRRRGLFREE
YQYSTLRENLGLPQPAINFANVKSA