Protein Info for IAI46_12065 in Serratia liquefaciens MT49

Annotation: peptidoglycan synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 572 transmembrane" amino acids 15 to 37 (23 residues), see Phobius details PF03717: PBP_dimer" amino acids 61 to 213 (153 residues), 64 bits, see alignment E=2.6e-21 PF00905: Transpeptidase" amino acids 253 to 548 (296 residues), 245.8 bits, see alignment E=5.8e-77

Best Hits

Swiss-Prot: 53% identical to FTSI_ECOLI: Peptidoglycan D,D-transpeptidase FtsI (ftsI) from Escherichia coli (strain K12)

KEGG orthology group: K03587, cell division protein FtsI (penicillin-binding protein 3) [EC: 2.4.1.129] (inferred from 96% identity to srs:SerAS12_2317)

MetaCyc: 53% identical to peptidoglycan DD-transpeptidase FtsI (Escherichia coli K-12 substr. MG1655)
Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.4.16.4]

Predicted SEED Role

"Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129)" in subsystem Bacterial Cell Division or Bacterial Cytoskeleton or Flagellum in Campylobacter or Peptidoglycan Biosynthesis (EC 2.4.1.129)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129, 3.4.16.4

Use Curated BLAST to search for 2.4.1.129 or 3.4.16.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (572 amino acids)

>IAI46_12065 peptidoglycan synthase (Serratia liquefaciens MT49)
MAPLTKKSGKNYSTLRFGWICGGILVCFFLLAFRVGYLQLLEHQQLADQADQRSIRTQVL
PTNRGMITDRNGEALAVSVSSKDIVLDPKHILDTHTDIGNERWQSLANVLKMPLPEVQRL
IQANAHKRFVYLARKVEDDNADYISKLRLTGVSTLQDFSRFYPMSQDAAGLIGIVGQDNQ
GLEGIELGFNHLLQGKNGLRVYQKDGSGAVIGVIKNVDPVPPPNVTLSIDKFIQYVLYAQ
IRDGVVANQADSGCAVLVKVNTGEVLGMASYPSFNPNNYANTPAKDIRNVCSSDSFEPGS
TVKPVVVMVGLEHRLIKPDTVLDTTPYRVNGHLIKDVGHWSKLTITGVLQKSSDIAVSHI
ALALPATVLPAVYKSFGLGRPTDLGIGNESSGYLPQHRERWADIERATFSFGYGLRVTPL
QMAREYAAIGSYGVYRPLSITRVTPPVMGQRILPEDTVKTVVHMMESDALPGGSGVSAAV
PGYRLAIKTGTAEKMGTNGKYDGGYINYTAGVAPASDPQVALVVMVNNPKAGKHFGGSVA
GPVFGKIMAQVLEHMNILPDAQPLNVVSQVKG