Protein Info for IAI46_11655 in Serratia liquefaciens MT49

Annotation: class II fumarate hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00979: fumarate hydratase, class II
  • amino acids 4 to 461 (458 residues), 810.5 bits, E=2.1e-248
• PF00206: Lyase_1
  • amino acids 12 to 342 (331 residues), 398.8 bits, E=2e-123
• PF10415: FumaraseC_C
  • amino acids 408 to 460 (53 residues), 88.3 bits, 3.5e-29

Best Hits

Swiss-Prot: 88% identical to FUMC_PHOLL: Fumarate hydratase class II (fumC) from Photorhabdus luminescens subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01)

KEGG orthology group: K01679, fumarate hydratase, class II [EC: 4.2.1.2] (inferred from 98% identity to spe:Spro_2279)

MetaCyc: 84% identical to fumarase C (Escherichia coli K-12 substr. MG1655)
Fumarate hydratase. [EC: 4.2.1.2]

Predicted SEED Role

"Fumarate hydratase class II (EC 4.2.1.2)" in subsystem TCA Cycle (EC 4.2.1.2)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• mixed acid fermentation (16/16 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle III (animals) (8/10 steps found)
• TCA cycle VIII (Chlamydia) (5/6 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (7/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (7/9 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• reductive TCA cycle I (8/11 steps found)
• incomplete reductive TCA cycle (5/7 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)
• reductive TCA cycle II (8/12 steps found)
• pyruvate fermentation to propanoate I (3/7 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (5/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (10/17 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)
• methylaspartate cycle (9/19 steps found)
• L-glutamate degradation VIII (to propanoate) (3/11 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (22/56 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.2

Use Curated BLAST to search for 4.2.1.2

Protein Sequence (465 amino acids)

>IAI46_11655 class II fumarate hydratase (Serratia liquefaciens MT49)
MAAVRIEKDSMGPIEVPADRLWGAQTQRSLEHFRISSEKMPTALIHALALTKRAAASVNM
DLGLLPAERANAIISAADEVLADQHTNEFPLSIWQTGSGTQTNMNMNEVLANRASELLGG
VRGEERRVHPNDDVNKSQSSNDVFPTAMHVAAVIAVREHLIPELKVLHKTLSAKAEAYRD
IVKIGRTHLQDATPLTLGQEISGWAAMLAHNLQHIEASIPHICELALGGTAVGTGLNTHP
EYAVRVAKALADLTRQPFVTAPNKFEALATCDALVHGHGALKGLAASLMKIANDVRWLSS
GPRCGIGEIAIPENEPGSSIMPGKVNPTQCEAMTMLCAQVLGNDVAVNIGGASGNFELNV
FRPMVIHNYLQSIRLLADGMQGFNEHCAVGIEPNRDRISQLLNESLMLVTALNTHIGYDK
AAEIAKKAHKEGLTLKASALKLGYLTEEQFDLWVRPEDMVGSMKK