Protein Info for IAI46_11365 in Serratia liquefaciens MT49

Annotation: tyrosine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 424 TIGR00234: tyrosine--tRNA ligase" amino acids 2 to 423 (422 residues), 469.3 bits, see alignment E=6.1e-145 PF00579: tRNA-synt_1b" amino acids 31 to 328 (298 residues), 299.6 bits, see alignment E=2.5e-93 PF01479: S4" amino acids 359 to 402 (44 residues), 25.4 bits, see alignment 9.5e-10

Best Hits

Swiss-Prot: 98% identical to SYY_SERP5: Tyrosine--tRNA ligase (tyrS) from Serratia proteamaculans (strain 568)

KEGG orthology group: K01866, tyrosyl-tRNA synthetase [EC: 6.1.1.1] (inferred from 98% identity to spe:Spro_2219)

MetaCyc: 84% identical to tyrosine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Tyrosine--tRNA ligase. [EC: 6.1.1.1]; 6.1.1.1 [EC: 6.1.1.1]

Predicted SEED Role

"Tyrosyl-tRNA synthetase (EC 6.1.1.1)" (EC 6.1.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (424 amino acids)

>IAI46_11365 tyrosine--tRNA ligase (Serratia liquefaciens MT49)
MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKR
FQLAGHKPVALVGGATGLIGDPSFKAAERKLNTTDTVNEWVEKIRKQVSPFLDFDCGNNS
AVAANNYDWFGGMNVLTFLRDIGKHFSVNQMINKEAVKQRLNRDDSGISFTEFSYNLLQG
FDFAELYNRHQVELQIGGSDQWGNITSGIDLTRRMHQQQVFGLTVPLITKADGTKFGKTE
GGAVWLAPEKTSPYKFYQFWINTADADVYRFLKFFTFMDLAEINALEEEDKNSGKAPRAQ
YVLAEEVTGMVHGAEGLAAAKRITQSLFSGALHDMTEADFAQLAQDGMPTIKLDGDADLQ
QALVNAELVPSRGQARTMIGSNAVTINGEKQSNAEYTFSDADRLFGRYTLLRRGKKHYCL
VDWQ