Protein Info for IAI46_11165 in Serratia liquefaciens MT49

Annotation: cysteine desulfurase SufS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01979: cysteine desulfurase, SufS family
  • amino acids 7 to 405 (399 residues), 608.7 bits, E=2.3e-187
• PF00266: Aminotran_5
  • amino acids 26 to 394 (369 residues), 532 bits, E=1.2e-163
• PF00155: Aminotran_1_2
  • amino acids 68 to 251 (184 residues), 34.8 bits, E=1.7e-12
• PF01212: Beta_elim_lyase
  • amino acids 71 to 245 (175 residues), 30.1 bits, E=4.6e-11

Best Hits

Swiss-Prot: 96% identical to SUFS_SERP5: Cysteine desulfurase (sufS) from Serratia proteamaculans (strain 568)

KEGG orthology group: K11717, cysteine desulfurase / selenocysteine lyase [EC: 2.8.1.7 4.4.1.16] (inferred from 96% identity to spe:Spro_2183)

MetaCyc: 71% identical to L-cysteine desulfurase (Escherichia coli K-12 substr. MG1655)
Selenocysteine lyase. [EC: 4.4.1.16]; Cysteine desulfurase. [EC: 4.4.1.16, 2.8.1.7]

Predicted SEED Role

"Cysteine desulfurase (EC 2.8.1.7), SufS subfamily" in subsystem Alanine biosynthesis (EC 2.8.1.7)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis I (10/10 steps found)
• superpathway of thiamine diphosphate biosynthesis II (10/11 steps found)
• thiazole component of thiamine diphosphate biosynthesis I (6/6 steps found)
• superpathway of L-alanine biosynthesis (4/4 steps found)
• thiazole component of thiamine diphosphate biosynthesis II (6/7 steps found)
• molybdopterin biosynthesis (5/6 steps found)
• L-alanine biosynthesis III (1/1 steps found)
• L-cysteine degradation IV (1/1 steps found)
• cytidylyl molybdenum cofactor sulfurylation (1/2 steps found)
• tRNA-uridine 2-thiolation and selenation (bacteria) (7/11 steps found)
• bis(guanylyl molybdopterin) cofactor sulfurylation (1/3 steps found)
• tRNA-uridine 2-thiolation (mammalian mitochondria) (1/4 steps found)
• tRNA-uridine 2-thiolation (yeast mitochondria) (1/4 steps found)
• tRNA-uridine 2-thiolation (thermophilic bacteria) (1/5 steps found)
• [2Fe-2S] iron-sulfur cluster biosynthesis (4/10 steps found)
• tRNA-uridine 2-thiolation (cytoplasmic) (1/8 steps found)

KEGG Metabolic Maps

• Selenoamino acid metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.7

Use Curated BLAST to search for 2.8.1.7 or 4.4.1.16

Protein Sequence (406 amino acids)

>IAI46_11165 cysteine desulfurase SufS (Serratia liquefaciens MT49)
MSFPIERVRGDFPILSREVNGQPLAYLDSAASAQKPQAVIDRELDFYRHGYAAVHRGIHT
MSAEATQEMEAVREKVAAFINAGSAEEIVFVKGTTEGINLVANSFGRHFLQPGDSIIITE
MEHHANIVPWQMLAQERGLNLRVWPLQQDGTLDVAQLPGLIDATTKLLALTQVSNVLGTV
NPVQEITAQAKAAGLKVLIDGAQAVMHQRVDVQALDCDFYVFSGHKLYGPSGIGVLYGRQ
ALLQQMPPWEGGGSMIQQVSLTLGTTYAEPPWRFEAGSPNTAGMMGLGAAIDYVNALGLE
AIADYEHSLMHYALEALQQVPTLKIYGPADRAGVIAFNLGEHHAYDVGSFLDQYGIAIRT
GHHCAMPLMAFYNVPSMCRASLALYNTREEVDRLVAGLQRIQKLLG