Protein Info for IAI46_10910 in Serratia liquefaciens MT49

Annotation: GMC family oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 593 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF00732: GMC_oxred_N" amino acids 234 to 347 (114 residues), 34.3 bits, see alignment E=2.2e-12 PF05199: GMC_oxred_C" amino acids 449 to 573 (125 residues), 75.6 bits, see alignment E=6e-25

Best Hits

Swiss-Prot: 82% identical to GADH1_PANCY: Gluconate 2-dehydrogenase flavoprotein from Pantoea cypripedii

KEGG orthology group: K06151, gluconate 2-dehydrogenase alpha chain [EC: 1.1.99.3] (inferred from 98% identity to spe:Spro_2137)

MetaCyc: 82% identical to gluconate 2-dehydrogenase dehydrogenase subunit (Pantoea cypripedii)
Gluconate 2-dehydrogenase (acceptor). [EC: 1.1.99.3]

Predicted SEED Role

"Gluconate 2-dehydrogenase (EC 1.1.99.3), membrane-bound, flavoprotein" in subsystem D-gluconate and ketogluconates metabolism (EC 1.1.99.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.3

Use Curated BLAST to search for 1.1.99.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (593 amino acids)

>IAI46_10910 GMC family oxidoreductase (Serratia liquefaciens MT49)
MATVMKKVDAVIVGFGWVGAIMAKELTEAGLNVVALERGPMRDTYPDGSYPQVIDELTYN
IRRKLFQDLSKSTVTIRHNTSQTAVPYRQLAAFLPGTGVGGAGLHWSGVHFRVDPIELRM
RSHYEERYGKSFIPKDMTIQDFGVSYAELEPFFDKAEKVFGTSGTAWTIKGQKVGQKGGN
PFAGDRSDDFPLPAQKNTFSAQLFEKAALEVGYHPYNLPSANTSDSYTNPYGAQMGPCNF
CGFCSGYACYMYSKASPNVNILPALRMEKRFELRTNANVLKVNLTADKSRATGVNYIDAQ
GREVEQPAELVILGAFQFHNVHLMLLSGIGKPYDPVTGEGVVGRNFAYQNMTTIKAFFDK
DVYTNPFIGAGGNGVGVDDFNADNFDHAKEGFVGGSPFWVNQAGTKPISGLPTPPGTPAW
GSKWKAAVADAYTHHVSMDAHGAHQSYRSNYLDLDPNYKNVFGQPLLRMTFDWQENDVKM
SQFMYDKMAPIAKAMNPKLIAGSPKNANSHFDTTSYQTTHMNGGAVMGEDPKTSAVNRYL
QSWDVHNVFSIGASAFPQGLGYNPTGTVAALAYWSARAIREQYLKNPGPLVQA