Protein Info for IAI46_10560 in Serratia liquefaciens MT49
Annotation: glutamine synthetase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 82% identical to PUUA_ECOLI: Gamma-glutamylputrescine synthetase PuuA (puuA) from Escherichia coli (strain K12)
KEGG orthology group: K09470, gamma-glutamylputrescine synthase [EC: 6.3.1.11] (inferred from 98% identity to spe:Spro_2068)MetaCyc: 82% identical to glutamate-putrescine ligase (Escherichia coli K-12 substr. MG1655)
Glutamate--putrescine ligase. [EC: 6.3.1.11]
Predicted SEED Role
"Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11)" (EC 6.3.1.11)
MetaCyc Pathways
- superpathway of L-arginine and L-ornithine degradation (11/13 steps found)
- superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (9/11 steps found)
- superpathway of ornithine degradation (6/8 steps found)
- putrescine degradation II (3/4 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.1.11
Use Curated BLAST to search for 6.3.1.11
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (472 amino acids)
>IAI46_10560 glutamine synthetase (Serratia liquefaciens MT49) MQTNIVEVENFVQHHEEKRSSAFQREVKKYLERYPLTQHVDVLLTDLNGSFRGKRIPVGG LSKLEKGCYFPASVFAMDILGNVVEEAGLGQELGEPDHICVPVLGTLTPSAADPQYIGQV LLTMLDEDGTPFDVEPRNVLNRVWQRLRQRGLSPVVAVELEFYLIDRQRDSEGYLQPPCA PGTQERNTQSQVYSVDNLNNFADVLSEIDELARLQGLPADGAVAEASPGQFEVNLHHTDN VLQACDHALALKRLIRMVAEKHNMQATFMAKPYEEHAGSGMHVHISMLDRQGNNVLADDD GEDSALLKQALAGMIALMPSSIALLAPNVNAYRRFQPGMYVPTQASWGHNNRTVALRIPC GDRDSHRVEYRVAGADANPYLVMATILAGIVYGLETPLPLQEPVTGNGLEQDGLPFPIRQ SDALYEFEHQPVLNALLGERFSHVYLACKTDELVQFERLITDTEIEWMLKNA