Protein Info for IAI46_10455 in Serratia liquefaciens MT49

Annotation: 23S rRNA pseudouridine(2457) synthase RluE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00849: PseudoU_synth_2
  • amino acids 31 to 176 (146 residues), 57 bits, E=1.4e-19
• TIGR00093: pseudouridine synthase
  • amino acids 35 to 206 (172 residues), 192.8 bits, E=1.7e-61

Best Hits

Swiss-Prot: 79% identical to RLUE_YERPE: Ribosomal large subunit pseudouridine synthase E (rluE) from Yersinia pestis

KEGG orthology group: K06181, ribosomal large subunit pseudouridine synthase E [EC: 5.4.99.12] (inferred from 91% identity to srs:SerAS12_1975)

MetaCyc: 75% identical to 23S rRNA pseudouridine2457 synthase (Escherichia coli K-12 substr. MG1655)
RXN-11834 [EC: 5.4.99.20]

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase E (EC 4.2.1.70)" (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (2/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12 or 5.4.99.20

Protein Sequence (208 amino acids)

>IAI46_10455 23S rRNA pseudouridine(2457) synthase RluE (Serratia liquefaciens MT49)
MKKFSVKNHKVKRFSKPAPAKPAVAQGPRRVVLFNKPFDVLPQFTDEAGRTTLKEFIPFP
DVYAAGRLDRDSEGLLVLTNDGKLQAQLTQPGKRTGKVYYVQVEGAPQESDLASLRNGVT
LKDGPTLPAGVELVAEPQWLWPRNPPIRERKSIPTAWLKVTLYEGRNRQVRRMTAHIGFP
TLRLIRYSMGNLSLGELQPGEWKTLDSL