Protein Info for IAI46_10440 in Serratia liquefaciens MT49

Annotation: tRNA 2-thiouridine(34) synthase MnmA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 367 PF02540: NAD_synthase" amino acids 4 to 76 (73 residues), 35.3 bits, see alignment E=2.4e-12 PF02568: ThiI" amino acids 5 to 76 (72 residues), 26.7 bits, see alignment E=1.4e-09 TIGR00420: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase" amino acids 7 to 361 (355 residues), 517.3 bits, see alignment E=8.3e-160 PF03054: tRNA_Me_trans" amino acids 7 to 203 (197 residues), 282.3 bits, see alignment E=7.5e-88 PF00733: Asn_synthase" amino acids 7 to 191 (185 residues), 28.5 bits, see alignment E=4.6e-10 PF20259: tRNA_Me_trans_M" amino acids 208 to 276 (69 residues), 82.7 bits, see alignment E=3.8e-27 PF20258: tRNA_Me_trans_C" amino acids 287 to 361 (75 residues), 93.6 bits, see alignment E=2.7e-30

Best Hits

Swiss-Prot: 98% identical to MNMA_SERP5: tRNA-specific 2-thiouridylase MnmA (mnmA) from Serratia proteamaculans (strain 568)

KEGG orthology group: K00566, tRNA-specific 2-thiouridylase [EC: 2.8.1.-] (inferred from 98% identity to spe:Spro_2022)

MetaCyc: 87% identical to tRNA-specific 2-thiouridylase (Escherichia coli K-12 substr. MG1655)
RXN0-2023 [EC: 2.8.1.13]

Predicted SEED Role

"tRNA-specific 2-thiouridylase MnmA"

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.-

Use Curated BLAST to search for 2.8.1.- or 2.8.1.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (367 amino acids)

>IAI46_10440 tRNA 2-thiouridine(34) synthase MnmA (Serratia liquefaciens MT49)
MSDNSQKKVIVGMSGGVDSSVTAYLLQQQGYQVAGLFMKNWEEDDDEEYCSAATDLADAQ
AVCDKLGIELHTVNFAAEYWDNVFELFLEEYKAGRTPNPDILCNKEIKFKAFLEFAAEDL
GADFIATGHYVRRQDVDGKSRLLRGVDGNKDQSYFLYTLSHEQVAQSLFPVGELEKPEVR
RIAQQLDLVTAKKKDSTGICFIGERKFRDFLGRYLPAQPGPIVSVDGQTVGEHQGLMYHT
LGQRKGLGIGGMKDSSEDPWYVVDKDVANNVLVVAQGHEHPRLMSVGLVAQQLHWVDRLP
LSGPFRCTVKTRYRQQDIPCTVTPLDQERIEVRFDEPVAAVTPGQSAVFYQDEICLGGGI
IEQRLQE