Protein Info for IAI46_10405 in Serratia liquefaciens MT49

Annotation: GMC family oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 592 PF00732: GMC_oxred_N" amino acids 235 to 345 (111 residues), 42.7 bits, see alignment E=6.1e-15 PF05199: GMC_oxred_C" amino acids 445 to 572 (128 residues), 65.4 bits, see alignment E=8.2e-22

Best Hits

Swiss-Prot: 59% identical to GADH1_PANCY: Gluconate 2-dehydrogenase flavoprotein from Pantoea cypripedii

KEGG orthology group: K06151, gluconate 2-dehydrogenase alpha chain [EC: 1.1.99.3] (inferred from 93% identity to spe:Spro_2015)

MetaCyc: 59% identical to D-gluconate dehydrogenase dehydrogenase subunit (Pseudomonas fluorescens)
Gluconate 2-dehydrogenase (acceptor). [EC: 1.1.99.3]

Predicted SEED Role

"Gluconate 2-dehydrogenase (EC 1.1.99.3), membrane-bound, flavoprotein" in subsystem D-gluconate and ketogluconates metabolism (EC 1.1.99.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.3

Use Curated BLAST to search for 1.1.99.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (592 amino acids)

>IAI46_10405 GMC family oxidoreductase (Serratia liquefaciens MT49)
MSTIKKPPVDVVIVGFGWTGAIMGMELTESGLSVLALERGEQRETYPDFAYPRIADELTY
GIRLKLAQEMAHETVTVRHTPADFAVPYRQLGSFLPGNGVGGAGVHWNGMHWRALPSDLK
LHTTLNEKYGAKWIPEGMTLQDYPLSYQELEPYFDKFEKVCGTSGRAGNLQGNIIKGGNP
FEGPRASEYPTPALKQLYSGSLFGKAAESLGYHPFAIPAANCSEPYTNPYGVQLGPCNFC
GYCERFGCFMYSKASPQTTILPVLTQRKNFELRTQSQVIKVNLDSTGKKATGVTYINAQG
QEVEQPADLVILSAFQLHNVRLLLLSGIGKPYDPVTGEGVVGKNYAYQMNSGVSLFYDQN
THFNPFIGAGAAGTVIDDLNGENFDHADLGFIGGAYISATRTGGRPIQQMMLPPGTPAWG
GGWKQGIKDNYPHSMSIGSEGSVMPYRDCYLDLDPTYKDGYGLPLLRMTFDWKDNEIKMT
QHITSKMVNIANAMNPKQIAVSVKKTGDHYDVRPYQSTHTTGGAITGDNPATSVVNKYLQ
CWDVPNVFVTGACAFPQNLAYNPTGIVGALAYYSAEAIRTQYLKNPGPLVQA