Protein Info for IAI46_10390 in Serratia liquefaciens MT49

Annotation: two-component system sensor histidine kinase PhoQ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 485 transmembrane" amino acids 15 to 40 (26 residues), see Phobius details amino acids 191 to 213 (23 residues), see Phobius details PF08918: PhoQ_Sensor" amino acids 8 to 186 (179 residues), 300.9 bits, see alignment E=7e-94 PF00512: HisKA" amino acids 267 to 328 (62 residues), 28.8 bits, see alignment E=2e-10 PF14501: HATPase_c_5" amino acids 375 to 462 (88 residues), 22.9 bits, see alignment E=1.4e-08 PF02518: HATPase_c" amino acids 375 to 476 (102 residues), 63.4 bits, see alignment E=5.2e-21

Best Hits

Swiss-Prot: 63% identical to PHOQ_SALTS: Virulence sensor histidine kinase PhoQ (phoQ) from Salmonella typhimurium (strain SL1344)

KEGG orthology group: K07637, two-component system, OmpR family, sensor histidine kinase PhoQ [EC: 2.7.13.3] (inferred from 98% identity to spe:Spro_2012)

MetaCyc: 64% identical to sensor histidine kinase PhoQ (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"Sensor protein PhoQ (EC 2.7.13.3)" in subsystem Lipid A modifications (EC 2.7.13.3)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (485 amino acids)

>IAI46_10390 two-component system sensor histidine kinase PhoQ (Serratia liquefaciens MT49)
MFNKGKKPFSLRARFLMATAGVILALSLSYGLVAVVGYIVSFDKTAFRLLRGESNLFFSL
AQWKDNKLTIAIPPDIDLNFPTLVFIYDDKGNLLWSQRKVPELEKRINKEWLEESGFYEI
DTDTRISSEVLGNNPKAQDQLKDYDDNDQNALTHSVAVNTYAATARLPALSIVVVDTIPQ
ELQRSDVVWEWFSYVLLANLLLVVPLLWLAAYWSLRPIKALVNQVGELENGEREQLDENP
PSELRGLVRNLNILVRNERQRYTKYRTTLSDLTHSLKTPLAVLQTTLRSLRTGKQTTIEE
VEPIMLDQISRISQQIGYYLHRASINSGQTVLTREIHSVPALLDSLNVALNKVYQRKGVV
ITLDISPEVTFMGEKNDFMEVMGNVLENACKYCLEFVEITSLHSDKYLTIVIDDDGPGIP
ESKRELIFQRGQRVDTLRPGQGLGLSVAAEIIEQYDGSITISDSPLGGARMQVTFARQHD
SHHHE