Protein Info for IAI46_10380 in Serratia liquefaciens MT49

Annotation: malate/lactate/ureidoglycolate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 363 PF02615: Ldh_2" amino acids 10 to 339 (330 residues), 374.7 bits, see alignment E=2e-116

Best Hits

Swiss-Prot: 66% identical to HCXB_ECOLI: Hydroxycarboxylate dehydrogenase B (hcxB) from Escherichia coli (strain K12)

KEGG orthology group: K13574, uncharacterized oxidoreductase [EC: 1.1.1.-] (inferred from 91% identity to spe:Spro_2010)

MetaCyc: 66% identical to hydroxycarboxylate dehydrogenase B (Escherichia coli K-12 substr. MG1655)
Hydroxyphenylpyruvate reductase. [EC: 1.1.1.237]

Predicted SEED Role

"Malate dehydrogenase (EC 1.1.1.37)" in subsystem Serine-glyoxylate cycle or TCA Cycle (EC 1.1.1.37)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.-, 1.1.1.37

Use Curated BLAST to search for 1.1.1.- or 1.1.1.237 or 1.1.1.37

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (363 amino acids)

>IAI46_10380 malate/lactate/ureidoglycolate dehydrogenase (Serratia liquefaciens MT49)
MSSTEHRIDRQCLEQFVQAIWRHAGSTEQEAARVADHLVQANLAGHDSHGVGMIPSYMSS
LAQGHLQLNVHAKVVRDAGAVLTVDGGQGFGQVVACEAMEKGIERARQLGLAAVALHNSH
HIGRIGHWAEQCARAGFISIHFVNVVGDPMVAPFGGSDRRFGTNPFCVIFPRKDKKPLLL
DFATSGIAFGKTRVAYNKGLSVAPGYLIDEHGQPTTEPKVMHEQPFGSLLPFGAHKGYAL
ATLCEILGGALSGGRTTHAATVKSNSDAIFNCMTTIILNPEAFDAPEMQTEAEAFIDWVK
ASPPSDGQPIEVPGEWEDANREARLRQGIPIDDNTWRQICAAAIQAGMSDAELDGYQTRA
LRA