Protein Info for IAI46_10280 in Serratia liquefaciens MT49

Annotation: peptide chain release factor 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 361 TIGR00019: peptide chain release factor 1" amino acids 1 to 357 (357 residues), 575.4 bits, see alignment E=2.1e-177 PF03462: PCRF" amino acids 12 to 203 (192 residues), 258.2 bits, see alignment E=5.1e-81 PF00472: RF-1" amino acids 216 to 321 (106 residues), 145.9 bits, see alignment E=4.8e-47

Best Hits

Swiss-Prot: 97% identical to RF1_SERP5: Peptide chain release factor 1 (prfA) from Serratia proteamaculans (strain 568)

KEGG orthology group: K02835, peptide chain release factor 1 (inferred from 98% identity to srs:SerAS12_1942)

Predicted SEED Role

"Peptide chain release factor 1" in subsystem LMPTP YwlE cluster

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (361 amino acids)

>IAI46_10280 peptide chain release factor 1 (Serratia liquefaciens MT49)
MKPSIVAKLEALQERHEEVQALLGDAGVIAEQDRFRALSREYAQLTDVSRCFLEWRQVQD
DLATAEMMLDDPEMREMAQEELKQAKAATEELEQQLQLLLLPKDPDDERGCFLEVRAGTG
GDEAAIFAGDLFRMYSRYAEMRRWRVEIMSANEGEHGGYKEVIAKVSGDGVYGQLKFESG
GHRVQRVPETESQGRIHTSACTVAVMPEIPEAELPEINAGDLKIDTFRSSGAGGQHVNTT
DSAIRITHLPTGIVVECQDERSQHKNKAKAMAVLGARIRAAEMAKRQQAEASTRRNLLGS
GDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVMEGKLDMLIQPIVQEYQADQLAALSAEQ
E