Protein Info for IAI46_09955 in Serratia liquefaciens MT49
Annotation: glyoxylate/hydroxypyruvate reductase GhrA
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 93% identical to GHRA_SERP5: Glyoxylate/hydroxypyruvate reductase A (ghrA) from Serratia proteamaculans (strain 568)
KEGG orthology group: K12972, gyoxylate/hydroxypyruvate reductase A [EC: 1.1.1.79 1.1.1.81] (inferred from 93% identity to spe:Spro_1933)MetaCyc: 60% identical to glyoxylate/hydroxypyruvate reductase A (Escherichia coli K-12 substr. MG1655)
Glyoxylate reductase (NADP(+)). [EC: 1.1.1.79]; 1.1.1.- [EC: 1.1.1.79]
Predicted SEED Role
"Glyoxylate reductase (EC 1.1.1.79) / Glyoxylate reductase (EC 1.1.1.26) / Hydroxypyruvate reductase (EC 1.1.1.81); 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215) (EC 1.1.1.-)" in subsystem Glycolate, glyoxylate interconversions (EC 1.1.1.-, EC 1.1.1.215, EC 1.1.1.26, EC 1.1.1.79, EC 1.1.1.81)
MetaCyc Pathways
- ketogluconate metabolism (8/8 steps found)
- L-serine biosynthesis II (3/4 steps found)
- formaldehyde assimilation I (serine pathway) (8/13 steps found)
- L-arabinose degradation IV (3/8 steps found)
- D-xylose degradation IV (2/7 steps found)
- superpathway of pentose and pentitol degradation (15/42 steps found)
KEGG Metabolic Maps
- Alkaloid biosynthesis I
- Aminosugars metabolism
- Ascorbate and aldarate metabolism
- Benzoate degradation via CoA ligation
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of type II polyketide products
- Biosynthesis of unsaturated fatty acids
- Bisphenol A degradation
- Butanoate metabolism
- C21-Steroid hormone metabolism
- Fructose and mannose metabolism
- Glycine, serine and threonine metabolism
- Glyoxylate and dicarboxylate metabolism
- Insect hormone biosynthesis
- Linoleic acid metabolism
- Nucleotide sugars metabolism
- Pentose phosphate pathway
- Polyketide sugar unit biosynthesis
- Pyruvate metabolism
- Retinol metabolism
- Tetrachloroethene degradation
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.-, 1.1.1.215, 1.1.1.81
Use Curated BLAST to search for 1.1.1.- or 1.1.1.215 or 1.1.1.26 or 1.1.1.79 or 1.1.1.81
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (313 amino acids)
>IAI46_09955 glyoxylate/hydroxypyruvate reductase GhrA (Serratia liquefaciens MT49) MNIIFYHPFFNAQDWLTGLSQRLPQATVRAWQPGDDRPADYALVWRPPYEMLANRGKLKG VFALGAGVDAILEQERQHPGTLPAGVPLVRLEDTGMAQQMQEYALSYVLRYFRRFDEYQL LQQQQKWQPLDPHQQENFTIGILGAGVLGKSVALKLADFGFNVRCWSRSPKQIAGVQSFA GAEQRAAFLDGVQLLINLLPNTPETAGILNQQLFAHLAPGAYLINLARGAHLVEDDLLQA LEQGQIAAATLDVFVEEPLPQAHPFWRHPRVTITPHIAAITLPEAAMDQIAANIRALESG QTPAGVVDVQLGY