Protein Info for IAI46_09120 in Serratia liquefaciens MT49

Annotation: 3-hydroxybutyryl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02737: 3HCDH_N
  • amino acids 10 to 188 (179 residues), 177.4 bits, E=5.5e-56
• PF03446: NAD_binding_2
  • amino acids 11 to 125 (115 residues), 23.6 bits, E=9.3e-09
• PF00725: 3HCDH
  • amino acids 191 to 286 (96 residues), 80 bits, E=3.2e-26
  • amino acids 421 to 494 (74 residues), 36.7 bits, E=1e-12
• PF18321: 3HCDH_RFF
  • amino acids 353 to 420 (68 residues), 37.1 bits, E=6.5e-13

Best Hits

KEGG orthology group: K00074, 3-hydroxybutyryl-CoA dehydrogenase [EC: 1.1.1.157] (inferred from 86% identity to spe:Spro_1832)

Predicted SEED Role

"3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)" (EC 1.1.1.157, EC 1.1.1.35)

MetaCyc Pathways

• oleate β-oxidation (29/35 steps found)
• fatty acid β-oxidation I (generic) (6/7 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• phenylacetate degradation I (aerobic) (7/9 steps found)
• (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (4/5 steps found)
• adipate biosynthesis (4/5 steps found)
• adipate degradation (4/5 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• pyruvate fermentation to hexanol (engineered) (8/11 steps found)
• superpathway of phenylethylamine degradation (8/11 steps found)
• fatty acid salvage (4/6 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
• glycerol degradation to butanol (11/16 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• pyruvate fermentation to butanoate (4/7 steps found)
• methyl ketone biosynthesis (engineered) (3/6 steps found)
• valproate β-oxidation (5/9 steps found)
• 4-hydroxybenzoate biosynthesis III (plants) (2/5 steps found)
• pyruvate fermentation to butanol I (4/8 steps found)
• benzoyl-CoA degradation I (aerobic) (3/7 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
• 2-methyl-branched fatty acid β-oxidation (8/14 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
• L-tryptophan degradation III (eukaryotic) (8/15 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (6/13 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (3/9 steps found)
• L-glutamate degradation VII (to butanoate) (5/12 steps found)
• 2-methylpropene degradation (2/8 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (9/18 steps found)
• 3-phenylpropanoate degradation (3/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (8/17 steps found)
• methyl tert-butyl ether degradation (2/10 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (3/16 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (3/17 steps found)
• toluene degradation VI (anaerobic) (3/18 steps found)
• cholesterol degradation to androstenedione I (cholesterol oxidase) (2/17 steps found)
• androstenedione degradation I (aerobic) (6/25 steps found)
• platensimycin biosynthesis (6/26 steps found)
• cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (2/22 steps found)
• superpathway of testosterone and androsterone degradation (6/28 steps found)
• androstenedione degradation II (anaerobic) (4/27 steps found)
• superpathway of cholesterol degradation I (cholesterol oxidase) (8/42 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (8/47 steps found)
• superpathway of cholesterol degradation III (oxidase) (4/49 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Bile acid biosynthesis
• Biosynthesis of plant hormones
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Lysine degradation
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.157, 1.1.1.35

Use Curated BLAST to search for 1.1.1.157 or 1.1.1.35

Protein Sequence (509 amino acids)

>IAI46_09120 3-hydroxybutyryl-CoA dehydrogenase (Serratia liquefaciens MT49)
MAENNTVINSVAVIGAGTMGRGIAYLLALNGIRTLLYNRSAHSLNQTRDYIVGDLNKKID
NGKISQQKKGEILANLVFSPVFDAIADSDLVIETIAEHEPTKHEILAAIAATVKKEAIIA
TNTSSLSLNKLAAGIENNSRFIGLHFFNPAPLMKLIEIIPSYFTARVTTLRCQQLVTAIG
KQFVVCKATPGFIVNRMARPFYLEGFRLLEENVALAPQIDRALKAGGHFRMGPLELTDFI
GQDINYQVSSQIWQDMQYDPRYTPGHLQRSLVDAGLLGKKNGRSFFSPSADTATPPAAGG
GTLSSLHFFGEHPLFDLLQQHALATWAPLPITRQPEHPVLGRFIQVNDSLAVKITDGRTA
NQLADLAGLDTFVIDVALNYANTAFLVAAHSQQATETNKALFLTLLHAVIPQVEFVKDSP
GLIVARVLSSLINESVIMVESGVCSREDIDIAAVAGVNYADGIFAWLAQLGQKNVKSTLD
NMAQLLHSARYYPHYSLLHTTRPELAVAP