Protein Info for IAI46_09045 in Serratia liquefaciens MT49

Annotation: pyrimidine utilization protein B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03614: pyrimidine utilization protein B
  • amino acids 22 to 241 (220 residues), 438.3 bits, E=3.1e-136
• PF00857: Isochorismatase
  • amino acids 37 to 234 (198 residues), 142.2 bits, E=9.7e-46

Best Hits

Swiss-Prot: 94% identical to RUTB_SERP5: Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB (rutB) from Serratia proteamaculans (strain 568)

KEGG orthology group: K09020, putative isochorismatase family protein RutB [EC: 3.-.-.-] (inferred from 94% identity to spe:Spro_1822)

MetaCyc: 83% identical to ureidoacrylate amidohydrolase / (+)-gamma-lactamase monomer (Escherichia coli K-12 JM109)
3.5.2.-; RXN0-6460 [EC: 3.5.1.110]

Predicted SEED Role

"Predicted amidohydrolase RutB in novel pyrimidine catabolism pathway" in subsystem Pyrimidine utilization

MetaCyc Pathways

• uracil degradation III (5/5 steps found)
• L-citrulline degradation (3/3 steps found)
• cyanate degradation (3/3 steps found)
• urea degradation I (3/3 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• superpathway of allantoin degradation in yeast (4/6 steps found)
• cyanuric acid degradation I (3/5 steps found)
• cyanuric acid degradation II (3/5 steps found)
• allantoin degradation IV (anaerobic) (4/9 steps found)
• superpathway of atrazine degradation (3/8 steps found)

KEGG Metabolic Maps

• Drug metabolism - other enzymes
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Puromycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 3.-.-.-

Use Curated BLAST to search for 3.-.-.- or 3.5.1.110

Protein Sequence (248 amino acids)

>IAI46_09045 pyrimidine utilization protein B (Serratia liquefaciens MT49)
MKIVESQPVVRRHSPQGGATVTLPARPEAIAFAPQETALIVVDMQNAYASQGGYLDLAGF
DVSTTAPVIANIKRAITAARAAGIKVIFFQNGWDNQYVEAGGEGSPNWHKSNALKTMRQR
PELMGKLLAKGDWDYDLVDELQPQPGDIVLPKPRYSGFFNTQLDSLLRSYGIHHLVFTGI
ATNVCVESTLRDGFFLEYFGIVLADATHQAGPAFAQQAALYNIETFFGWVSDVDSFCDVL
AAPLSQTA