Protein Info for IAI46_08970 in Serratia liquefaciens MT49

Annotation: GMC family oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 551 PF00732: GMC_oxred_N" amino acids 245 to 330 (86 residues), 25.1 bits, see alignment E=2.1e-09 PF05199: GMC_oxred_C" amino acids 419 to 539 (121 residues), 71.9 bits, see alignment E=1.3e-23

Best Hits

Swiss-Prot: 54% identical to FDHL_GLUJA: Fructose dehydrogenase large subunit (fdhL) from Gluconobacter japonicus

KEGG orthology group: None (inferred from 97% identity to spe:Spro_1808)

MetaCyc: 54% identical to fructose dehydrogenase large subunit (Gluconobacter japonicus)
FRUCTOSE-5-DEHYDROGENASE-RXN [EC: 1.1.5.14]

Predicted SEED Role

"2-Keto-D-gluconate dehydrogenase (EC 1.1.99.4), membrane-bound, flavoprotein" in subsystem D-gluconate and ketogluconates metabolism (EC 1.1.99.4)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.4

Use Curated BLAST to search for 1.1.5.14 or 1.1.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (551 amino acids)

>IAI46_08970 GMC family oxidoreductase (Serratia liquefaciens MT49)
MKKPEFTADGNASADIVIVGSGIVGGMMADQLVSQGYSVLVLEAGLRIERAQAVENWRNM
PFDNRAGSDYQGLYPQSEFATAPLYFPENNYVALSGPSAGSFKQGYLRTVGGTTWHWAAS
CWRHLPSDFQMKTLYGVGRDWPISYDELEPYYCRAEEEIGVAGPNDPQQQSPVERSKPYP
MDMVPWAHGDIRFAEVVNPHGYRSVPIPQGRSTHPWKGRPTCCGNNNCQPICPIGAMYNG
IHHVERAEMKGAVVLAEAVVYKIDTDEQNRVTAVHWLDNKKQSHQATAKAFALACNGIET
PRLLLMAANDRNPNGIANASDQVGRNMMDHSGFHCTFLAKEPLWLGRGPAQSSCLVGPRD
GEFRKDYSANKMILNNINRVVPATQKALEQGLLGKELDAEIRRRAAYGVDLSISLEPLPD
PNNRLTLSKTRKDAHGLPCPDIHYDVGEYVRKGAEAAHKQLEHIGQLFDAEEFNITTSLN
ANNHIMGGTIMGDNPADSVVDGNCRTHDHANLWLPGGGAIPSASVVNSTLTMAALGIKAA
DDIARALAVKP