Protein Info for IAI46_08820 in Serratia liquefaciens MT49

Annotation: bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2))-methyltransferase RlmL

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 706 PF02926: THUMP" amino acids 24 to 153 (130 residues), 64.1 bits, see alignment E=7.7e-21 PF01170: UPF0020" amino acids 162 to 375 (214 residues), 207.7 bits, see alignment E=7.2e-65 PF10672: Methyltrans_SAM" amino acids 439 to 659 (221 residues), 72 bits, see alignment E=2.5e-23 PF03602: Cons_hypoth95" amino acids 532 to 639 (108 residues), 41.6 bits, see alignment E=5.4e-14

Best Hits

Swiss-Prot: 87% identical to RLMKL_PECAS: Ribosomal RNA large subunit methyltransferase K/L (rlmL) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K12297, ribosomal RNA large subunit methyltransferase L [EC: 2.1.1.173] (inferred from 82% identity to ddc:Dd586_1642)

MetaCyc: 76% identical to fused 23S rRNA m2G2445 methyltransferase and 23S rRNA m7G2069 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-6950 [EC: 2.1.1.264]; RXN-11574 [EC: 2.1.1.264, 2.1.1.173]

Predicted SEED Role

"23S rRNA (guanine-N-2-) -methyltransferase rlmL EC 2.1.1.-)"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.173 or 2.1.1.264

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (706 amino acids)

>IAI46_08820 bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2))-methyltransferase RlmL (Serratia liquefaciens MT49)
MNSLFASTARGLEELLKSELEALGAHACKVVQGGVHFQGDDRLLYQSLLWSRLASRILLP
LNEFRVHSDLDLYLGVQAIDWTNIFGVDKTFAVHFSGVNEEIRNSQYGALKVKDAIVDSF
TRKLDQRPTVAKQQPDIRVNVFLQRDMASVALDLSGEGLHQRGYRDLTGQAPLKESLAAA
IVQRSGWQPGTPMLDPMCGSGTLLIEAAMIASDRAPGLHRQHWGFTAWNGHNVDLWREVI
TEAQVRARRGLQETTSRFFGSDIDRRVIEMARGNARRAGVAELITFNVNDVAKLTNPLPE
GPTGTVVCNPPYGERLESEPALIALHNMLGRIMKSAFGGWQLSLFSASPELLSCLQLRAE
RQFKAKNGPLDCVQKNYQLAENPQGSGGIQVAEDFANRLRKNLKKLDKWAKQQGIECYRL
YDADLPEYNVAVDRYGSKVVVQEYAPPKTIDAQKARQRLFDVINATLAVLELPSNQLILK
TRERQKGKNQYEKLAQKGEFLLVEEFNAKLWVNLTDYLDTGLFLDHRIARRMLGEMSKGK
DFLNLFAYTGTASVHAGLGGARSTTTVDMSRTYLEWAEKNLRVNGLTGKQHRLIHADCLS
WLQNADEQFDVIFIDPPTFSNSKRMENTFDVQRDHLVLMQDLKRLLRRNGTIMFSNNKRG
FQMDMAGLSALGLEAKEITAQTQSQDFARNRQIHNCWLVTHAGEGK