Protein Info for IAI46_08325 in Serratia liquefaciens MT49

Annotation: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01048: PNP_UDP_1
  • amino acids 27 to 272 (246 residues), 136 bits, E=6.9e-44

Best Hits

KEGG orthology group: K01243, S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase [EC: 3.2.2.9] (inferred from 89% identity to spe:Spro_1636)

Predicted SEED Role

"5'-methylthioadenosine nucleosidase (EC 3.2.2.16) / S-adenosylhomocysteine nucleosidase (EC 3.2.2.9)" in subsystem Adenosyl nucleosidases or Methionine Salvage or Polyamine Metabolism or Methionine Biosynthesis or Methionine Degradation (EC 3.2.2.16, EC 3.2.2.9)

MetaCyc Pathways

• L-methionine salvage cycle I (bacteria and plants) (11/12 steps found)
• S-adenosyl-L-methionine salvage I (4/4 steps found)
• L-methionine salvage cycle II (plants) (9/11 steps found)
• autoinducer AI-2 biosynthesis II (Vibrio) (5/6 steps found)
• S-methyl-5'-thioadenosine degradation I (2/2 steps found)
• autoinducer AI-2 biosynthesis I (4/5 steps found)
• S-methyl-5'-thioadenosine degradation IV (1/1 steps found)
• 5'-deoxyadenosine degradation II (3/4 steps found)
• L-cysteine biosynthesis VI (reverse transsulfuration) (5/7 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.9

Use Curated BLAST to search for 3.2.2.16 or 3.2.2.9

Protein Sequence (278 amino acids)

>IAI46_08325 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (Serratia liquefaciens MT49)
MNLVLLAVCGALFSPLTVAQQGVVPGPIVVQGAMPVEAERFAQRLDNPREEQIGGWRFWR
GTVDGYPVVVSETLKGMSNSAAATAIAATEFRPVAIINQGTAGGHDPALKVYDIVLGKYS
VSLGAFKTPKKALGEGSDSRQWQPMDLLASKGSAGEDKNPHTIRQFPADAKLLAIAESVK
GSYAQGKVVEGVIGSADVWNSELDRIRDFREKYHTSVEEMETASAAQIAAAFNIPFVGIR
ALSNNITNHGKYDPQTGLACQDYVYQVVKAYIAENASR