Protein Info for IAI46_08160 in Serratia liquefaciens MT49

Annotation: NADP-dependent phosphogluconate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00873: 6-phosphogluconate dehydrogenase (decarboxylating)
  • amino acids 5 to 468 (464 residues), 760.8 bits, E=2.6e-233
• PF03446: NAD_binding_2
  • amino acids 5 to 173 (169 residues), 173 bits, E=8.1e-55
• PF00393: 6PGD
  • amino acids 178 to 466 (289 residues), 451.8 bits, E=1.7e-139

Best Hits

Swiss-Prot: 88% identical to 6PGD_ECOLI: 6-phosphogluconate dehydrogenase, decarboxylating (gnd) from Escherichia coli (strain K12)

KEGG orthology group: K00033, 6-phosphogluconate dehydrogenase [EC: 1.1.1.44] (inferred from 98% identity to spe:Spro_1608)

MetaCyc: 88% identical to 6-phosphogluconate dehydrogenase, decarboxylating (Escherichia coli K-12 substr. MG1655)
Phosphogluconate dehydrogenase (decarboxylating). [EC: 1.1.1.44]

Predicted SEED Role

"6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44)" in subsystem Pentose phosphate pathway (EC 1.1.1.44)

MetaCyc Pathways

• superpathway of glucose and xylose degradation (16/17 steps found)
• pentose phosphate pathway (8/8 steps found)
• pentose phosphate pathway (oxidative branch) I (3/3 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Glutathione metabolism
• Pentose phosphate pathway

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.44

Protein Sequence (468 amino acids)

>IAI46_08160 NADP-dependent phosphogluconate dehydrogenase (Serratia liquefaciens MT49)
MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSGDKTDEVIAENPGKNLVPHYTVEEF
VESLEKPRRILLMVKAGEATDKTIASLTPHLDKGDILIDGGNTYYQDTIRRNRELSDQGF
NFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILEKIAAVAEGEPCVTYIGADGAGHY
VKMVHNGIEYGDMQLIAEAYSLLKQALNLSNEELADTFAEWNKGELNSYLIDITKDIFTK
KDEEGKYLVDVILDEAANKGTGKWTSQSSLDLGEPLSLITESVFARYLSSLKDQRVAASK
VLTGPKVTPVSGDKAEFVEKVRRALYLGKIVSYAQGFSQLKAASKENNWDLHYGDIAKIF
RAGCIIRAQFLQKITDAYTQDADIANLLLAPYFKQIADEYQQALRDVVSYAVQNGIPTPT
FSAAISYYDSYRSAVLPANLIQAQRDYFGAHTYKRIDKEGVFHTEWME