Protein Info for IAI46_08070 in Serratia liquefaciens MT49

Annotation: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01479: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase
  • amino acids 8 to 472 (465 residues), 771.4 bits, E=1.6e-236
• PF00483: NTP_transferase
  • amino acids 10 to 290 (281 residues), 227.4 bits, E=4.3e-71
• PF12804: NTP_transf_3
  • amino acids 11 to 136 (126 residues), 29.7 bits, E=1.4e-10
• PF01050: MannoseP_isomer
  • amino acids 320 to 470 (151 residues), 241.8 bits, E=5.4e-76
• PF07883: Cupin_2
  • amino acids 386 to 453 (68 residues), 41.2 bits, E=2.3e-14

Best Hits

Swiss-Prot: 65% identical to MANC_SALTY: Mannose-1-phosphate guanylyltransferase ManC (manC) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00971, mannose-1-phosphate guanylyltransferase [EC: 2.7.7.22] (inferred from 67% identity to rah:Rahaq_3592)

MetaCyc: 55% identical to phosphomannose isomerase / GDP-D-mannose pyrophosphorylase (Pseudomonas aeruginosa)
Mannose-6-phosphate isomerase. [EC: 5.3.1.8]; Mannose-1-phosphate guanylyltransferase. [EC: 5.3.1.8, 2.7.7.13]

Predicted SEED Role

"Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22)" in subsystem Alginate metabolism or Mannose Metabolism (EC 2.7.7.22)

MetaCyc Pathways

• GDP-mannose biosynthesis (4/4 steps found)
• D-mannose degradation I (2/2 steps found)
• D-mannose degradation II (2/2 steps found)
• mannitol degradation II (3/4 steps found)
• colanic acid building blocks biosynthesis (8/11 steps found)
• β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation (2/3 steps found)
• mannitol biosynthesis (2/3 steps found)
• 1,5-anhydrofructose degradation (3/5 steps found)
• β-(1,4)-mannan degradation (2/7 steps found)
• superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (6/14 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.3.1.8

Use Curated BLAST to search for 2.7.7.13 or 2.7.7.22 or 5.3.1.8

Protein Sequence (475 amino acids)

>IAI46_08070 mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase (Serratia liquefaciens MT49)
MRVKMSNLYPVIMAGGTGSRLWPLSRELFPKQFLALCNEFSMLQTTVMRLKGLDIINPLV
ICNEEHRFIVAEQLRQITRLSHNIILEPVGRNTAPAIALAALQAVSSGDDPLMLVLAADH
VIQDEATFREAVNQAVPYAEAGKLATFGIVPTGPETGYGYIQKGTSVDGSSICGVSRFVE
KPNLETAQQYLDSGEYLWNSGMFLFKASRYLDELERFRPDILDACKKSLAHLSPDMDFIR
VDREAFIACPDESVDYAVMEQTADAVVVPLDAGWNDVGSWSALWEISEKDVKGNSTFGDV
LEHDCSNNYIRAEHKLVAMVGIDNLVVVETKDAVLIANKDKVQDVKEIVNQLKQQKRSES
KQHREVYRPWGKHDAIAQGERFQVRRITVKPGEKLSLQMHHHRSEHWVVVSGTAKVNANG
KVQLISENESIYIPLGTEHSLENPGKIELDLIEIQSGAYLGEDDIVRIGDSAQHN