Protein Info for IAI46_08030 in Serratia liquefaciens MT49

Annotation: N-acetylneuraminate synthase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 746 PF00571: CBS" amino acids 12 to 55 (44 residues), 23.4 bits, see alignment 1.2e-08 PF03102: NeuB" amino acids 150 to 384 (235 residues), 279.3 bits, see alignment E=5.2e-87 PF08666: SAF" amino acids 403 to 458 (56 residues), 42.2 bits, see alignment 1.9e-14 PF01261: AP_endonuc_2" amino acids 491 to 744 (254 residues), 91.4 bits, see alignment E=1.6e-29

Best Hits

KEGG orthology group: K01654, N-acetylneuraminate synthase [EC: 2.5.1.56] (inferred from 69% identity to mme:Marme_0612)

Predicted SEED Role

"N-acetylneuraminate synthase (EC 2.5.1.56)" in subsystem CMP-N-acetylneuraminate Biosynthesis or Sialic Acid Metabolism (EC 2.5.1.56)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.56

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (746 amino acids)

>IAI46_08030 N-acetylneuraminate synthase family protein (Serratia liquefaciens MT49)
MLIQKKISDFLVFETETIIKALEKINSNKKRIIFVVTEDGKLCGSFSDGDFRRWITSGDF
DLSREMSEVMNQNVRYKKVDAPRTEIEAFFTNGVDVLPLVDDYRRLVAVALQSEKGLTIG
KHTITDTSPSYIIAEIGNNHNGDIKLAKHLVDLAVDAGADCVKFQMRDLSSLYKGGNEKD
QSADLGAQYTIDLLRKFQLSNEELIEVFDYCKTKGLTPLCTPWDLRSLEILEQYGMEGYK
VASADFTNHEMLGALAATGKPLICSTGMCSEAEIKSSVEFLKSKGAEFVILHCNSTYPTP
FKDVNLAYIKRLQQVTGCLVGYSGHERGISVPIAVAALGARVIEKHFTIDKSMEGNDHKV
SLLPPEFKEMVVRIREVEESMGQAGERTITQGEMINRETLAKSLIINRDLKKGEIITRDM
IEVKSPGQGLQPLYMEQLVGKKAIRDLEAGGYFYESDLNDEAIEAKDYDFDRPFGIPVRY
HDYSKLKNKSNLDFVEFHLSYQDMELDLTEFFSGAEDIGFAVHSPELFANDHILDLASED
PAYLAHSIKQLQRVCDVTRALKQYFPKTECPVIVVNAGGFNTTGFLPKEARQGMYERIGS
ALSQINQEGVEIIIQTMPPFPWHFGGQSYHNLFVDPDEIKAFCEKYNYRICYDTSHSMMA
CNYYQWDLHEFTQKVGAYTAHMHVVDALGVDGEGVQIGKGDVNFDELADDLLRYAPGAMF
IPEVWQGHKNQGEGFWHALNFLEKYL