Protein Info for IAI46_07880 in Serratia liquefaciens MT49

Annotation: S-formylglutathione hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02821: S-formylglutathione hydrolase
  • amino acids 6 to 278 (273 residues), 394 bits, E=2e-122
• PF00756: Esterase
  • amino acids 26 to 261 (236 residues), 222.7 bits, E=9.5e-70
• PF00326: Peptidase_S9
  • amino acids 131 to 261 (131 residues), 33 bits, E=6.7e-12

Best Hits

Swiss-Prot: 72% identical to SFGH2_ECO24: S-formylglutathione hydrolase YeiG (yeiG) from Escherichia coli O139:H28 (strain E24377A / ETEC)

KEGG orthology group: K01070, S-formylglutathione hydrolase [EC: 3.1.2.12] (inferred from 98% identity to spe:Spro_1556)

MetaCyc: 72% identical to S-formylglutathione hydrolase / S-lactoylglutathione hydrolase (Escherichia coli K-12 substr. MG1655)
Hydroxyacylglutathione hydrolase. [EC: 3.1.2.6]; S-formylglutathione hydrolase. [EC: 3.1.2.6, 3.1.2.12]

Predicted SEED Role

"S-formylglutathione hydrolase (EC 3.1.2.12)" in subsystem Glutathione-dependent pathway of formaldehyde detoxification (EC 3.1.2.12)

MetaCyc Pathways

• formaldehyde oxidation II (glutathione-dependent) (3/3 steps found)
• superpathway of methylglyoxal degradation (6/8 steps found)
• methylglyoxal degradation I (2/3 steps found)
• methylglyoxal degradation VIII (2/3 steps found)
• superpathway of C1 compounds oxidation to CO₂ (4/12 steps found)

KEGG Metabolic Maps

• Methane metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.2.6

Use Curated BLAST to search for 3.1.2.12 or 3.1.2.6

Protein Sequence (280 amino acids)

>IAI46_07880 S-formylglutathione hydrolase (Serratia liquefaciens MT49)
MTLSLELLEEHRMFGGWQQRYRHAAQSLNCNMTFSIYLPPPRDDNPPPVLYWLSGLTCND
ENFTLKAGAQRIAAELGLVLVMADTSPRGDDVPNDEGYDLGQGAGFYLNATQAPWDKHFR
MYDYISDELPALINQHFSVSDRQSIFGHSMGGHGALMMAFRNPQRFRSVSAFAPIVNPCQ
VPWGRKAFAAYLGDDESQWLQYDSCHLLANGAEKMPVLVDQGDDDQFLADQLQPAKLAEL
ARQRDWPLILRIQPGYDHSYFTIASFIEDHLRFHAEHLFR