Protein Info for IAI46_07510 in Serratia liquefaciens MT49

Annotation: 23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF05971: Methyltransf_10
  • amino acids 10 to 308 (299 residues), 426.5 bits, E=3e-132

Best Hits

Swiss-Prot: 98% identical to RLMF_SERP5: Ribosomal RNA large subunit methyltransferase F (rlmF) from Serratia proteamaculans (strain 568)

KEGG orthology group: K06970, ribosomal RNA large subunit methyltransferase F [EC: 2.1.1.181] (inferred from 98% identity to spe:Spro_1470)

MetaCyc: 55% identical to 23S rRNA m6A1618 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11596 [EC: 2.1.1.181]

Predicted SEED Role

"Ribosomal RNA large subunit methyltransferase F (EC 2.1.1.51)" (EC 2.1.1.51)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.51

Use Curated BLAST to search for 2.1.1.181 or 2.1.1.51

Protein Sequence (321 amino acids)

>IAI46_07510 23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF (Serratia liquefaciens MT49)
MEKKKTFPQQKSGLHPRNRHRSRYDFPALIASCPALEPFVKPNAWGDISVDFADPAAVKM
LNRALLQHFYGIEHWDIPADYLCPPIPGRADYLHHLADLLATSNGGEIPRGKGVAILDVG
IGANCIYPIVGLREYGWRFTGSEIDPVSLNSAKMIVEMNPTLRNSVRLRLQKQPEFIFNG
IIGVAEKFDATLCNPPFHGSEQEAQASTRRKLHKLGKGEVADKPVQNFGGKNNELWCEGG
EEAFVRKMVEESVSKAQNCLWFTSLISKNTTLPSIYHALKLAGAAEVRTIEMAQGQKISR
FVAWTFHDAEQQAAWAAERWR