Protein Info for IAI46_06525 in Serratia liquefaciens MT49

Annotation: biotin synthase BioB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00433: biotin synthase
  • amino acids 14 to 310 (297 residues), 412.4 bits, E=5.5e-128
• PF04055: Radical_SAM
  • amino acids 48 to 205 (158 residues), 82 bits, E=5.7e-27
• PF06968: BATS
  • amino acids 220 to 311 (92 residues), 106.3 bits, E=7.6e-35

Best Hits

Swiss-Prot: 98% identical to BIOB_SERP5: Biotin synthase (bioB) from Serratia proteamaculans (strain 568)

KEGG orthology group: K01012, biotin synthetase [EC: 2.8.1.6] (inferred from 98% identity to spe:Spro_1311)

MetaCyc: 88% identical to biotin synthase (Escherichia coli K-12 substr. MG1655)
Biotin synthase. [EC: 2.8.1.6]

Predicted SEED Role

"Biotin synthase (EC 2.8.1.6)" in subsystem Biotin biosynthesis (EC 2.8.1.6)

MetaCyc Pathways

• biotin biosynthesis I (13/15 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate I (4/4 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate II (3/4 steps found)
• biotin biosynthesis II (4/6 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate III (3/5 steps found)

KEGG Metabolic Maps

• Biotin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.6

Protein Sequence (345 amino acids)

>IAI46_06525 biotin synthase BioB (Serratia liquefaciens MT49)
MADRIHWTVGLAQTLFDKPLLELLFEAQTVHRQHFDPRQVQVSTLLSIKTGACPEDCKYC
PQSSRYKTGLESERLMQVEQVLESARKAKANGSTRFCMGAAWKNPHERDMPYLQQMVQGV
KAMGMETCMTLGTLDGSQAERLAEAGLDYYNHNLDTSPEFYGNIITTRSYQERLDTLGKV
RGAGIKVCSGGIVGLGETVRDRAGLLVQLANLPTPPESVPINMLVKVKGTPLADNDDVDP
FDFIRTIAVARIMMPSSYVRLSAGREQMNEQTQAMCFMAGANSIFYGCKLLTTPNPEEDK
DLQLFRKLGLNPQQTETEHGDNQQQEVLAARLMQADTAEFYNAAL