Protein Info for IAI46_06275 in Serratia liquefaciens MT49

Annotation: 2-oxoglutarate dehydrogenase E1 component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF16078: 2-oxogl_dehyd_N
  • amino acids 12 to 49 (38 residues), 57.5 bits, see alignment (E = 1.6e-19)
• TIGR00239: oxoglutarate dehydrogenase (succinyl-transferring), E1 component
  • amino acids 14 to 932 (919 residues), 1578.7 bits, E=0
• PF00676: E1_dh
  • amino acids 226 to 512 (287 residues), 153.8 bits, E=1.1e-48
• PF02779: Transket_pyr
  • amino acids 592 to 785 (194 residues), 176.1 bits, E=1e-55
• PF16870: OxoGdeHyase_C
  • amino acids 790 to 932 (143 residues), 184.1 bits, E=2.7e-58

Best Hits

Swiss-Prot: 88% identical to ODO1_ECOLI: 2-oxoglutarate dehydrogenase E1 component (sucA) from Escherichia coli (strain K12)

KEGG orthology group: K00164, 2-oxoglutarate dehydrogenase E1 component [EC: 1.2.4.2] (inferred from 88% identity to esc:Entcl_3101)

MetaCyc: 88% identical to subunit of E1(0) component of 2-oxoglutarate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Oxoglutarate dehydrogenase (succinyl-transferring). [EC: 1.2.1.105, 1.2.4.2]

Predicted SEED Role

"2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2)" in subsystem TCA Cycle (EC 1.2.4.2)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• 2-oxoglutarate decarboxylation to succinyl-CoA (3/3 steps found)
• TCA cycle III (animals) (8/10 steps found)
• TCA cycle VIII (Chlamydia) (5/6 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• 2-amino-3-carboxymuconate semialdehyde degradation to glutaryl-CoA (3/5 steps found)
• L-tryptophan degradation III (eukaryotic) (8/15 steps found)
• L-tryptophan degradation XI (mammalian, via kynurenine) (8/23 steps found)
• superpathway of L-lysine degradation (13/43 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Lysine degradation
• Tryptophan metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.105 or 1.2.4.2

Protein Sequence (937 amino acids)

>IAI46_06275 2-oxoglutarate dehydrogenase E1 component (Serratia liquefaciens MT49)
MQNGAMKAWLDSSYLAGANQSYIEQLYEDFLTDPGSVEDSWRSIFQQLPTAGVKPDQLHS
QTRDYFRRLAKDSARYNTTINDPDTDAKQVKVLQLINAFRFRGHQHANLDPLGLWQREQV
PDLEPAYHNLTEADFQETFNVGSFAIGKETMKLGDLYAALKQTYCGSIGAEYMHITNTEE
KRWIQQRIESVVGHASFTDDEKRRFLNELTAAEGLERYLGAKFPGAKRFSLEGGDALVPM
LKEMVRHAGKNGTREVVLGMAHRGRLNVLINVLGKKPADLFDEFAGKHKEHLGTGDVKYH
QGFSSDVETEGGMVHLALAFNPSHLEIVSPVVMGSVRARRDRLDEARSNMVLPITIHGDA
AITGQGVVQETLNMSQARGYEVGGTVRIVINNQVGFTTSNPLDARSTEYCTDIAKMVQSP
IFHVNADDPEAVAFVTRLALDFRNTFKRDVMIDLVCYRRHGHNEADEPSATQPVMYQKIK
KHPTPRKLYADVLTEQKVASLEDATEMVNLYRDALDRGDCVVEEWRPMNLHSFTWSPYLN
HEWDEEYPSKVEMKRLQELARRISTVPEAIEMQSRVAKIYADRAEMAAGNKAFDWGAAET
LAYATMVDEGIPIRLSGEDAGRGTFFHRHAVVHNQKNGSVYVPLANVHSGQGEFKVWDSV
LSEEAVLAFEYGYATAEPRTLTIWEAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLL
PHGYEGQGPEHSSARLERYLQLCAEQNMQVCIPSTPAQVYRMLRRQALRGMRRPLVVMSP
KSLLRHPLATSSLEELANGTFLPAIGEIDELDPKAVKRVVMCSGKVYYDLLEQRRKNDQK
DVAIIRIEQLYPFPHQAVQAVLEKYAHVHDFVWCQEEPLNQGAWYCSQHNFREVVPFGAS
LRYAGRPASASPAVGYMSVHQKQQQALVNDALNIVKD