Protein Info for IAI46_06165 in Serratia liquefaciens MT49

Annotation: two-component system sensor histidine kinase KdbD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 897 transmembrane" amino acids 400 to 421 (22 residues), see Phobius details amino acids 427 to 444 (18 residues), see Phobius details amino acids 450 to 467 (18 residues), see Phobius details amino acids 478 to 499 (22 residues), see Phobius details PF02702: KdpD" amino acids 23 to 231 (209 residues), 323.8 bits, see alignment E=1.3e-100 PF13493: DUF4118" amino acids 404 to 509 (106 residues), 108 bits, see alignment E=6.2e-35 PF13492: GAF_3" amino acids 570 to 646 (77 residues), 46.7 bits, see alignment E=1.3e-15 PF00512: HisKA" amino acids 666 to 732 (67 residues), 48.5 bits, see alignment 2.3e-16 PF02518: HATPase_c" amino acids 777 to 885 (109 residues), 96.9 bits, see alignment E=3.1e-31

Best Hits

Swiss-Prot: 70% identical to KDPD_ECOLI: Sensor protein KdpD (kdpD) from Escherichia coli (strain K12)

KEGG orthology group: K07646, two-component system, OmpR family, sensor histidine kinase KdpD [EC: 2.7.13.3] (inferred from 72% identity to enc:ECL_03023)

MetaCyc: 70% identical to sensor histidine kinase KdpD (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)" in subsystem Potassium homeostasis (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (897 amino acids)

>IAI46_06165 two-component system sensor histidine kinase KdbD (Serratia liquefaciens MT49)
MVDEEQQRPDPDSLLALANEKPRGRLKVFFGACAGVGKTYAMLQEAQRLRSQGLDVLVGV
VETHGRSETAALLNGLDLLAQKRIQHRGRLVQEFDLDAALARHPALILMDELAHSNAYGS
RHPKRWQDVEELLDAGIDVLTTVNVQHLESLNDVVGGVTGVRVRETVPDHVFDEATEVVL
VDLPPDDLRQRLNEGKVYIPGQAERAIEHFFRKGNLIALRELALRRTADRVDDQMRAFRD
TQGRERVWHTRDSILLCVGHSSGNEKLVRIAARLAARLGCHWHAVYVETPKLHRLPENQR
RAILRALRLAQELGAETATLSEPYEERAVLRYAREHNLGKIIIGRRSEQRWKLRGSFADR
LGRLGPDLDLVIVALENDTPAPQGKEHDGRNFTEKWRMQLRGCAVAVALCALITLLSQWL
LPGFDQANLVMVYLLGVAIVALFFGRWPSVLAAVINVASFDLFFVQPEWSFAVSDMQYLL
TFGVMLIVGITIGNLTAGVRYQARVARYREQRARHLYEISRGLSQALTTEDIAKTSRYFL
SSSFQAKTVLLLPQEDGKLQQMVGETGGLLSVDEAIARWSFDKGMPAGAGTDTLPGVPYQ
LLPLTTSKQTFGLLAIEPSNLRQLMVPEQQRLLQTFAVLIASALERLHLARSAEEAKLDA
EREQLRNSLLAALSHDLRTPLTVLFGQAEILTLDLAAEGSNHAPQASQIRQQVLSTTRLV
NNLLDMARIQSGGFNLRKEWQSLEEIVGASLHMLEPLMTQHQIKVELPPELILVNCDGSL
LERVFTNLLENANKYAGIDATIGIRAHTLPDWLEVEVWDDGPGIPEGKKQLIFDKFSRGN
KESAIPGVGLGLAICRAIIEVHGGRIWAENGANGGASFRFVLPLEKPPEIDSEAFDL